RNAi-mediated allelic trans-interaction at the imprinted Rtl1/Peg11 locus
RNAi-mediated allelic trans-interaction at the imprinted Rtl1/Peg11 locus
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DOI：
10.1016/j.cub.2005.02.060
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发表时间：
2005-04-26
期刊：
影响因子：
9.2
通讯作者：
Charlier, C
中科院分区：
文献类型：
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作者：
Davis, E;Caiment, F;Charlier, C
Dlk1-Gtl2印迹结构域，包括绵羊的Callipyge(CLPG)基因，最近被证明含有大量母体表达的miRNA基因[1，2]。其中两个(mir127和mir136)是从转录本(AntiPeg11)中加工而来的，该转录本与Rtl1/Peg11反义，Rtl1/Peg11是父系表达的无内含子基因，与寿司样反转录元件的Gag和Pol多蛋白同源[3]。在这里，我们证明了几个额外的miRNAs是从antiPeg11加工而来的，这些miRNAs通过引导RISC介导的对其mRNA的切割来调节TRANS中的Rtl1/Peg11。这是第一次证明miRNA介导的RNAi涉及哺乳动物的印记基因。
The Dlk1-Gtl2 imprinted domain, encompassing the callipyge (CLPG) locus in sheep, has recently been shown to harbor a large number of maternally expressed miRNA genes [1, 2]. Two of these (mir127 and mir136) are processed from a transcript (antiPeg11) that is antisense to Rtl1/Peg11, a paternally expressed intronless gene with homology to the gag and pol polyproteins of Sushi-like retroelements [3]. We herein demonstrate that several additional miRNAs are processed from antiPeg11 and that these regulate Rtl1/Peg11 in trans by guiding RISC-mediated cleavage of its mRNA. This is the first demonstration of miRNA-mediated RNAi involving imprinted genes in mammals.