PD-1-expressing MAIT cells from patients with tuberculosis exhibit elevated production of CXCL13
PD-1-expressing MAIT cells from patients with tuberculosis exhibit elevated production of CXCL13
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来自结核病患者的表达 PD-1 的 MAIT 细胞表现出 CXCL13 产量升高
DOI：
10.1111/sji.12858
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发表时间：
2020-01-08
影响因子：
3.7
通讯作者：
Cheng, Xiaoxing
中科院分区：
文献类型：
--
作者：
Jiang, Jing;Cao, Zhihong;Cheng, Xiaoxing
为了理解表达PD-1的MAIT细胞在人类结核病感染期间的功能作用，对来自胸膜结核病患者的胸腔积液的分选的PD-1（+）和PD-1 ㈠ MAIT细胞进行转录组测序。通过流式细胞术分析表达PD-1的MAIT细胞，并研究其表型和功能特征。转录组测序鉴定了144个在来自结核性胸腔积液的PD-1（+）和PD-1（-）MAIT细胞之间差异表达的基因，并且CXCL 13是具有最高倍数差异的基因。表达PD-1的MAIT细胞的水平与人类TB感染的程度相关。通过流式细胞术测定，表达PD-1的MAIT细胞具有增加的CXCL 13和IL-21的产生。与来自结核病患者和健康对照的外周血的那些相比，来自胸膜结核病患者的胸腔积液中的PD-1（高）CXCR 5（-）MAIT细胞显著扩增。尽管来自结核性胸腔积液的PD-1（高）CXCR 5（-）MAIT细胞具有降低的IFN-γ水平和增加的Tim-3和GITR表达，但它们显示活化的表型，并且具有较高的葡萄糖摄取和脂质含量。得出结论，表达PD-1的MAIT细胞具有降低的IFN-γ水平，但增加了CXCL 13和IL-21的产生。
To understand functional role of PD-1-expressing MAIT cells during tuberculosis infection in humans, sorted PD-1(+) and PD-1(-) MAIT cells from pleural effusions of patients with pleural tuberculosis were subjected to transcriptome sequencing. PD-1-expressing MAIT cells were analysed by flow cytometry and their phenotypic and functional features were investigated. Transcriptome sequencing identified 144 genes that were differentially expressed between PD-1(+) and PD-1(-) MAIT cells from tuberculous pleural effusions and CXCL13 was the gene with highest fold difference. The level of PD-1-expressing MAIT cells was associated with extent of TB infection in humans. PD-1-expressing MAIT cells had increased production of CXCL13 and IL-21 as determined by flow cytometry. PD-1(high)CXCR5(-) MAIT cells were significantly expanded in pleural effusions from patients with pleural tuberculosis as compared with those from peripheral blood of both patients with tuberculosis and healthy controls. Although PD-1(high)CXCR5(-) MAIT cells from tuberculous pleural effusions had reduced IFN-gamma level and increased expression of Tim-3 and GITR, they showed activated phenotype and had higher glucose uptake and lipid content. It is concluded that PD-1-expressing MAIT cells had reduced IFN-gamma level but increased production of both CXCL13 and IL-21.