Guidance of primordial germ cell migration by the chemokine SDF-1
Guidance of primordial germ cell migration by the chemokine SDF-1
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DOI：
10.1016/s0092-8674(02)01135-2
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发表时间：
2002-11-27
期刊：
影响因子：
64.5
通讯作者：
Raz, E
中科院分区：
文献类型：
--
作者：
Doitsidou, M;Reichman-Fried, M;Raz, E
在脊椎动物中指导原始生殖细胞（PGC）迁移的信号在很大程度上是未知的。我们表明，SDF-1 mRNA表达的位置，PGC被发现，并朝着他们在野生型以及在突变体胚胎中的PGC迁移是异常的。敲低SDF-1或其受体CXCR 4导致PGC迁移的严重缺陷。具体地说，未接收SDF-1信号的PGC表现出缺乏朝向其靶点的定向运动，并到达胚胎内的异位位置。最后，我们表明，PGC可以被吸引到一个异位源的趋化因子，强烈表明，这种分子提供了一个关键的方向提示PGC。
The signals directing primordial germ cell (PGC) migration in vertebrates are largely unknown. We demonstrate that sdf-1 mRNA is expressed in locations where PGCs are found and toward which they migrate in wild-type as well as in mutant embryos in which PGC migration is abnormal. Knocking down SDF-1 or its receptor CXCR4 results in severe defects in PGC migration. Specifically, PGCs that do not receive the SDF-1 signal exhibit lack of directional movement toward their target and arrive at ectopic positions within the embryo. Finally, we show that the PGCs can be attracted toward an ectopic source of the chemokine, strongly suggesting that this molecule provides a key directional cue for the PGCs.