Synaptic connections between motor neurons and interneurons in the fourth thoracic ganglion of the crayfish, Procambarus clarkii.
Synaptic connections between motor neurons and interneurons in the fourth thoracic ganglion of the crayfish, Procambarus clarkii.
复制标题
克氏原螯虾第四胸神经节运动神经元和中间神经元之间的突触连接。
作者：
A. Chrachri;F. Clarac
1.一种新的制备胸神经系统的小龙虾，克氏原螯虾，已经开发出来，其中它是可能的工作与确定的成员的运动神经元池。2.在这样的准备中，可以解剖所有特定的近端运动神经（量角器、牵开器、前电梯、后电梯和降压器）。通过微电极用荧光黄对记录的运动神经元进行染色，从生理学和解剖学两个方面鉴定了支配第四条步行腿四个近端肌肉的运动神经元。3.通过使用氯化钴，我们绘制了第四胸神经节内所有运动神经元胞体的分布，这些神经元支配控制第四步行腿运动的不同肌肉群。4.大多数支配同一肌肉的运动神经元似乎是电耦合的，除了一些降压运动神经元。5.支配拮抗肌的运动神经元通过抑制性连接连接。这些连接是相互的量肌和牵开肌运动神经元，但通常不是电梯和降压运动神经元之间的相互关系。6.行走中间神经元被鉴定为在任何运动神经中没有轴突的神经元，其改变了运动神经元的活动。他们中的一些人被注射了路西法黄。7.一些中间神经元只与控制一个关节运动的拮抗运动神经元建立突触连接。可能它们的功能作用是加强或限制每对拮抗运动神经元之间的拮抗作用。8.其他中间神经元与支配控制不同腿关节的肌肉的运动神经元形成突触连接。这些中间神经元可能在产生向前和向后行走的运动模式中发挥作用。
1. A new preparation of the thoracic nervous system of the crayfish, Procambarus clarkii, has been developed, in which it is possible to work with identified members of motor neuronal pools. 2. In such a preparation, it is possible to dissect all specific proximal motor nerves (protractor, retractor, anterior elevator, posterior elevator, and depressor). Motor neurons innervating the four proximal muscles of the fourth walking leg have been identified both physiologically and anatomically by staining the recorded motor neuron with Lucifer yellow through the microelectrode. 3. By the use of cobalt chloride, we have mapped the distribution of somata of all motor neurons within the fourth thoracic ganglion that innervate the different groups of muscles controlling the movement of the fourth walking leg. 4. Most motor neurons innervating the same muscle seem to be electrically coupled, except some depressor motor neurons. 5. Motor neurons innervating antagonist muscles are linked by inhibitory connections. These connections are reciprocal for protractor and retractor motor neurons but usually not reciprocal between elevator and depressor motor neurons. 6. Walking interneurons were identified as neurons without axons in any motor nerve, which modified the motor neuronal activity. Some of them have been injected with Lucifer yellow. 7. Some interneurons make synaptic connections only with antagonist motor neurons that control the movement of one joint. Probably their functional role is to reinforce or to limit the antagonism between each pair of antagonist motor neurons. 8. Other interneurons make synaptic connections with motor neurons innervating muscles controlling different leg joints. These interneurons may play a role in generating the motor patterns that underlie forward and backward walking.