Protective Effect of Molecular Hydrogen Following Different Routes of Administration on D-Galactose-Induced Aging Mice.
Protective Effect of Molecular Hydrogen Following Different Routes of Administration on D-Galactose-Induced Aging Mice.
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DOI：
10.2147/jir.s332286
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发表时间：
2021
影响因子：
4.5
通讯作者：
Qin S
中科院分区：
文献类型：
--
作者：
Liu B;Xie Y;Chen J;Xue J;Zhang X;Zhao M;Jia X;Wang Y;Qin S
分子氢（H₂）已被公认为是一种有效的抗氧化剂，几乎没有副作用。虽然已知氧化应激与衰老密切相关，但H₂对氧化应激相关衰老的有益作用仍不明确。在本研究中，采用D - 半乳糖诱导衰老的小鼠模型来探究H₂的保护作用。
通过不同途径（吸入4% H₂、饮用富氢水和注射富氢生理盐水）给小鼠施用H₂，然后检测血浆中与衰老相关的生物标志物以及不同组织中的氧化应激情况。
结果显示，H₂改善了血浆中与衰老相关的生物标志物，即总抗氧化能力、晚期糖基化终末产物、肿瘤坏死因子 - α、游离脂肪酸和丙氨酸氨基转移酶。此外，H₂通过降低脂质过氧化水平和丙二醛含量、提高超氧化物歧化酶活性，减轻了肝脏、大脑和心脏中的氧化应激。另外，就H₂的摄入量以及降低某些组织中的氧化应激标志物而言，吸入4% H₂似乎最为有效；不过，其他给药途径在大多数指标上也产生了相同的效果。
通过不同途径给药时，H₂能够预防D - 半乳糖诱导衰老小鼠的氧化应激。
Molecular hydrogen (H2) has been recognized as an effective antioxidant with no or little side effects. While it is known that oxidative stress is closely associated with aging, the beneficial effect of H2 on oxidative stress-related aging is still unclear. In this study, a mouse model of D-galactose-induced aging was employed to investigate the protective effects of H2. The mice were administrated of H2 via different routes (4% H2 inhalation, H2-rich water drinking, and H2-rich saline injection), the aging-related biomarkers in plasma and the oxidative stress in different tissues were measured. The results showed that H2 improved aging-related biomarkers, ie, total antioxidant capacity, advanced glycation end products, tumor necrosis factor-α, free fatty acids, and alanine aminotransferase in plasma. Furthermore, H2 alleviated oxidative stress in the liver, brain, and heart by reducing the levels of lipid peroxidation and malondialdehyde and increasing the activity of superoxide dismutase. In addition, it seems that 4% H2 inhalation was the most effective regarding the amount of H2 taken up and in reducing the markers of oxidative stress in some of the tissues; however, the other routes of administration resulted in the same efficacy in most indicators. H2 can prevent oxidative stress in D-galactose-induced aging mice when administered by different routes.