The kiss/kissr Systems Are Dispensable for Zebrafish Reproduction: Evidence From Gene Knockout Studies
The kiss/kissr Systems Are Dispensable for Zebrafish Reproduction: Evidence From Gene Knockout Studies
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Kiss/Kissr 系统对于斑马鱼的繁殖是可有可无的：来自基因敲除研究的证据。
DOI：
10.1210/en.2014-1204
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发表时间：
2015-02-01
期刊：
影响因子：
4.8
通讯作者：
Cheng, Christopher H. K.
中科院分区：
文献类型：
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作者：
Tang, Haipei;Liu, Yun;Cheng, Christopher H. K.
kiss1/gpr54信号系统被认为是大多数脊椎动物繁殖的关键调节因子。然而，这一假设尚未在非哺乳动物脊椎动物中得到有力的检验。与哺乳动物不同，在非哺乳动物脊椎动物中发现了多个kiss1/gpr54旁系基因（kiss/kissr），这提高了这些基因之间功能冗余的可能性。在本研究中，我们系统地生成了斑马鱼的kiss1（-/-）、kiss2（-/-）和kiss1(-/-)；Kiss2（-/-）突变系以及kissr1（-/-）、kissr2（-/-）和kissr1(-/-)；使用转录激活子样效应核酸酶的Kissr2（-/-）突变系。我们已经证明，在这6个突变系中，精子发生和卵泡发生以及生殖能力都没有受到损害。总的来说，我们的研究结果表明，亲吻/亲吻信号对于斑马鱼的繁殖并不是绝对必需的，这表明亲吻/亲吻系统在某些非哺乳动物的繁殖中起着非必要的作用。这些发现还表明，鱼类和哺乳动物进化出了不同的生殖神经内分泌控制策略。
The kiss1/gpr54 signaling system is considered to be a critical regulator of reproduction in most vertebrates. However, this presumption has not been tested vigorously in nonmammalian vertebrates. Distinct from mammals, multiple kiss1/gpr54 paralogous genes (kiss/kissr) have been identified in nonmammalian vertebrates, raising the possibility of functional redundancy among these genes. In this study, we have systematically generated the zebrafish kiss1(-/-), kiss2(-/-), and kiss1(-/-);kiss2(-/-) mutant lines as well as the kissr1(-/-), kissr2(-/-), and kissr1(-/-);kissr2(-/-) mutant lines using transcription activator-like effector nucleases. We have demonstrated that spermatogenesis and folliculogenesis as well as reproductive capability are not impaired in all of these 6 mutant lines. Collectively, our results indicate that kiss/kissr signaling is not absolutely required for zebrafish reproduction, suggesting that the kiss/kissr systems play nonessential roles for reproduction in certain nonmammalian vertebrates. These findings also demonstrated that fish and mammals have evolved different strategies for neuroendocrine control of reproduction.