GLYCOSYL RECEPTORS IN MACROPHAGE SUBPOPULATIONS OF RAT SPLEEN AND LYMPH-NODE - A COMPARATIVE-STUDY USING NEOGLYCOPROTEINS AND MONOCLONAL-ANTIBODIES ED1, ED2 AND ED3
GLYCOSYL RECEPTORS IN MACROPHAGE SUBPOPULATIONS OF RAT SPLEEN AND LYMPH-NODE - A COMPARATIVE-STUDY USING NEOGLYCOPROTEINS AND MONOCLONAL-ANTIBODIES ED1, ED2 AND ED3
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DOI：
10.1007/bf00327742
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发表时间：
1990-10-01
影响因子：
3.6
通讯作者：
HARDONK, MJ
中科院分区：
文献类型：
--
作者：
HARMS, G;DIJKSTRA, CD;HARDONK, MJ
我们建立了一种免疫组织化学方法，利用新糖蛋白在体内和体外检测大鼠脾脏和淋巴结的糖基受体。这两个器官的受体都能识别甘露糖偶联的牛血清白蛋白（甘露糖- bsa）、牛毛糖- bsa、n -乙酰氨基葡萄糖- bsa和较小程度的葡萄糖- bsa，但不能识别半乳糖- bsa或n -乙酰半乳糖- bsa。在体外，新糖蛋白与受体的结合依赖于Ca2+，可以被甘露糖抑制，而不是被甘露糖抑制。同时用单克隆抗体ED1、ED2或ED3染色显示，只有ED1和ED3阳性的巨噬细胞参与了新糖蛋白的结合。在脾脏中，边缘区巨噬细胞和边缘金属细胞亚群具有糖基结合受体。在淋巴结中，髓窦巨噬细胞和外皮层巨噬细胞亚群能够结合新糖蛋白。
We have developed an immunohistochemical method for the in vivo and in vitro detection of glycosyl receptors in rat spleen and lymph nodes by using neoglycoproteins. The receptor in both organs recognized mannose coupled to bovine serum albumin (mannose-BSA), fuscose-BSA, N-acetylglucosamine-BSA and to a lesser extent glucose-BSA, but not galactose-BSA or N-acetylgalactosamine-BSA. In vitro neoglycoprotein-receptor bindings was Ca2+ dependent and could be inhibited by mannan but not by mannose. Simultaneous staining with the monoclonal antibodies ED1, ED2 or ED3 revealed that only ED1- and ED3-positive macrophages were involved in the binding of neoglycorpoteins. In the spleen, the marginal-zone macrophages and a subpopulation of the marginal metallophils possess glycosyl-binding receptors. In the lymph nodes, the medullary sinus macrophages and a subpopulation of the outer-cortex macrophages are able to bind neoglycoproteins.