A RhoA-derived peptide inhibits syncytium formation induced by respiratory syncytial virus and parainfluenza virus type 3
A RhoA-derived peptide inhibits syncytium formation induced by respiratory syncytial virus and parainfluenza virus type 3
复制标题
DOI：
10.1038/71503
复制
发表时间：
2000-01-01
期刊：
影响因子：
82.9
通讯作者：
Graham, BS
中科院分区：
文献类型：
--
作者：
Pastey, MK;Gower, TL;Graham, BS
人呼吸道合胞病毒（RSV）和人副流感病毒3型（PIV-3）的融合糖蛋白介导病毒进入和合胞体形成。RSV与RhoA（一种小的G蛋白酶）的融合蛋白之间的相互作用促进病毒诱导的合胞体形成。我们在此显示RhoA衍生肽抑制RSV和由RSV和PIV-3诱导的合胞体形成，在体外通过抑制细胞间融合，在体内通过在RSV感染的小鼠中将峰值滴度降低2 log（10）。这些发现表明，这两个副粘病毒融合蛋白和RhoA之间的相互作用是新的抗病毒策略的重要靶标。
The fusion glycoproteins of human respiratory syncytial virus (RSV) and human parainfluenza virus type-3 (PIV-3) mediate virus entry and syncytium formation. Interaction between the fusion protein of RSV and RhoA, a small GTPase, facilitates virus-induced syncytium formation. We show here a RhoA-derived peptide inhibits RSV and syncytium formation induced by RSV and PIV-3, both in vitro by inhibition of cell-to-cell fusion and in vivo by reduction of peak titer by 2 log(10) in RSV-infected mice. These findings indicate that the interaction between these two paramyxovirus fusion proteins and RhoA is an important target for new antiviral strategies.