Mapping osmotic adjustment in an advanced back-cross inbred population of rice
Mapping osmotic adjustment in an advanced back-cross inbred population of rice
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DOI：
10.1007/s00122-003-1360-7
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发表时间：
2003-11-01
影响因子：
5.4
通讯作者：
Li, Z
中科院分区：
文献类型：
--
作者：
Robin, S;Pathan, MS;Li, Z
渗透调节是与水稻耐旱性有关的几个特征之一。已知籼稻品种比粳稻品种具有更大的渗透调节能力。我们利用籼稻供体IR 62266 -42-6-2，将渗透调节功能基因导入粳稻优良品种IR 60080 - 46 A，构建了一个高级回交群体。150 BC 3F 3家庭的基因型使用微卫星和RFLP标记，和一些候选基因。我们评估了在温室条件下，使用再水化技术在这些线的渗透调节。利用复合区间作图技术，共检测到14个QTL，分别位于第1、2、3、4、5、7、8和10染色体上，共解释了58%的表型变异。大多数（但不是全部）具有积极影响的等位基因来自供体父母。在第8染色体上，有两个与排斥相关的QTL。QTL定位与以前在水稻和其他谷物中对该性状的研究结果一致。一些BC 3F 3株系在两个标记上携带了最多4个QTL的有利等位基因。将这些品系进行杂交，然后在其后代中进行标记辅助选择，这对于恢复具有与供体亲本相等的渗透调节水平的品系是必要的。先进的回交策略似乎是一个合适的方法，以加快过程中的渗入感兴趣的性状到精英材料。
Osmotic adjustment is one of several characters putatively associated with drought tolerance in rice. Indica cultivars are known to have a greater capacity for osmotic adjustment than japonica cultivars. We developed an advanced back-cross population using an indica donor, IR62266-42-6-2, to introgress osmotic adjustment into an elite japonica cultivar, IR60080-46A. One hundred and fifty BC3F3 families were genotyped using microsatellites and RFLP markers, and a few candidate genes. We evaluated osmotic adjustment in these lines under greenhouse conditions using the re-hydration technique. Using the composite interval mapping technique, we detected 14 QTLs located on chromosomes 1, 2, 3, 4, 5, 7, 8 and 10 that together explained 58% of the phenotypic variability. Most, but not all, of the alleles with positive effects came from the donor parent. On chromosome 8, two QTLs were associated in repulsion. The QTL locations were in good agreement with previous studies on this trait on rice and in other cereals. Some BC3F3 lines carried the favorable alleles at the two markers flanking up to four QTLs. Intercrossing these lines followed by marker-aided selection in their progenies will be necessary to recover lines with levels of osmotic adjustment equal to the donor parent. The advanced back-cross strategy appeared to be an appropriate method to accelerate the process of introgressing interesting traits into elite material.