A spatiotemporal molecular atlas of the ovulating mouse ovary.
A spatiotemporal molecular atlas of the ovulating mouse ovary.
复制标题
排卵小鼠卵巢的时空分子图。
DOI：
10.1073/pnas.2317418121
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发表时间：
2024-01-30
影响因子：
11.1
通讯作者：
De Vlaminck, Andiwijn
中科院分区：
文献类型：
--
作者：
Mantri, Madhav;Zhang, Hanxue Hannah;Spanos, Emmanuel;Ren, Yi A.;De Vlaminck, Andiwijn
排卵失败占美国育龄妇女不孕症病例的10%以上。因此，迫切需要更好地了解排卵。然而，排卵的机制是非常困难的研究，因为排卵是一个高度动态的过程，在卵巢内的小空间局限的壁龛中以分钟的时间尺度运行。在这篇手稿中，我们应用高空间和时间分辨率的空间转录组学来生成小鼠卵巢中细胞转换和细胞-细胞相互作用的分子图谱。该图谱提供了对调节小鼠卵泡成熟和排卵的复杂过程的见解，对促进生殖医学的治疗策略具有重要意义。排卵是生殖成功的关键，但潜在的细胞和分子机制还远不清楚。在这里，我们应用高分辨率时空转录组学来绘制细胞类型和排卵阶段特异性分子程序，作为小鼠卵泡成熟和排卵期间时间的函数。我们的分析揭示了颗粒细胞类型内的动态分子转换与间充质细胞增殖密切相关。我们确定了在胃窦前到胃窦过渡期出现的卵丘细胞命运的分子标记。我们描述了快速响应排卵刺激和与卵泡破裂相关的转录程序，突出了在卵泡成熟的最后阶段凋亡和代谢途径的重要作用。我们进一步报告了特定阶段的卵母细胞-卵丘细胞的相互作用和接近排卵的卵泡的分化分子。总的来说，这项研究提供了对调节小鼠卵泡成熟和排卵的细胞和分子过程的深入了解，对推进生殖医学的治疗策略具有重要意义。
Ovulation failure accounts for over 10% of infertility cases in women of reproductive age in the United States. There is therefore an urgent need for a better understanding of ovulation. Yet, the mechanisms of ovulation are exceptionally difficult to study given that ovulation is a highly dynamic process that operates on a time scale of minutes in small spatially confined niches within the ovary. In this manuscript, we apply spatial transcriptomics at high spatial and temporal resolution to generate a molecular atlas of cellular transitions and cell–cell interactions in the mouse ovary. This atlas provides insights into intricate processes that regulate mouse ovarian follicle maturation and ovulation with important implications for advancing therapeutic strategies in reproductive medicine. Ovulation is essential for reproductive success, yet the underlying cellular and molecular mechanisms are far from clear. Here, we applied high-resolution spatiotemporal transcriptomics to map out cell type– and ovulation stage–specific molecular programs as function of time during follicle maturation and ovulation in mice. Our analysis revealed dynamic molecular transitions within granulosa cell types that occur in tight coordination with mesenchymal cell proliferation. We identified molecular markers for the emerging cumulus cell fate during the preantral-to-antral transition. We describe transcriptional programs that respond rapidly to ovulation stimulation and those associated with follicle rupture, highlighting the prominent roles of apoptotic and metabolic pathways during the final stages of follicle maturation. We further report stage-specific oocyte–cumulus cell interactions and diverging molecular differentiation in follicles approaching ovulation. Collectively, this study provides insights into the cellular and molecular processes that regulate mouse ovarian follicle maturation and ovulation with important implications for advancing therapeutic strategies in reproductive medicine.
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影响因子：
4.8
作者：
Leu, SJ;Lam, SCT;Lau, LF
通讯作者：
Lau, LF
影响因子：
4.8
作者：
Choi, Yohan;Jeon, Hayce;Jo, Misung
通讯作者：
Jo, Misung
DOI：
10.1093/bioinformatics/btac757
发表时间：
2023-01-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1530/rep-08-0457
发表时间：
2009-05
期刊：
Reproduction (Cambridge, England)
影响因子：
--
作者：
Carletti MZ;Christenson LK
通讯作者：
Christenson LK
影响因子：
4.6
作者：
Cacioppo JA;Lin PP;Hannon PR;McDougle DR;Gal A;Ko C
通讯作者：
Ko C