Nanomechanical properties of the sea-water bacterium Paracoccus seriniphilus--a scanning force microscopy approach.
Nanomechanical properties of the sea-water bacterium Paracoccus seriniphilus--a scanning force microscopy approach.
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DOI：
10.1116/1.4906862
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发表时间：
2015-02
期刊：
影响因子：
2.1
通讯作者：
N. Davoudi;C. Müller‐Renno;C. Ziegler;Indek Raid;J. Seewig;C. Schlegel;K. Muffler;R. Ulber
中科院分区：
文献类型：
--
作者：
N. Davoudi;C. Müller‐Renno;C. Ziegler;Indek Raid;J. Seewig;C. Schlegel;K. Muffler;R. Ulber
单个细菌上的力-距离曲线的测量提供了一个独特的机会来检测各种环境条件下的特性，如膨胀压力。海洋细菌是非常有趣的药物生产候选者，但到目前为止，人们对其研究还很少。因此，本研究对产酶海洋生物丝状副球藻的弹性行为进行了研究。在仔细评估最佳测量条件后，弹簧常数和膨胀压力被确定为离子强度和pH的函数。而离子强度对膨压的影响是被动的，结果表明，向酸性pH的变化是通过一种积极的机制增加了膨压。此外，可以看出，丝虫在其细胞壁外有粘附性突起。
The measurement of force-distance curves on a single bacterium provides a unique opportunity to detect properties such as the turgor pressure under various environmental conditions. Marine bacteria are very interesting candidates for the production of pharmaceuticals, but are only little studied so far. Therefore, the elastic behavior of Paracoccus seriniphilus, an enzyme producing marine organism, is presented in this study. After a careful evaluation of the optimal measurement conditions, the spring constant and the turgor pressure are determined as a function of ionic strength and pH. Whereas the ionic strength changes the turgor pressure passively, the results give a hint that the change to acidic pH increases the turgor pressure by an active mechanism. Furthermore, it could be shown, that P. seriniphilus has adhesive protrusions outside its cell wall.