The function of reovirus proteins during the reovirus multiplication cycle: analysis using monoreassortants.
The function of reovirus proteins during the reovirus multiplication cycle: analysis using monoreassortants.
复制标题
呼肠孤病毒蛋白在呼肠孤病毒增殖周期中的功能：使用单重配体进行分析。
DOI：
10.1016/0042-6822(89)90556-4
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发表时间：
1989
期刊：
影响因子：
3.7
通讯作者：
Joklik,WK
中科院分区：
文献类型：
--
作者：
Moody,MD;Joklik,WK
当用两种呼肠孤病毒株的核心的混合物注射培养的细胞时，高比例的重组体是单重组体，即含有1个亲本的一个基因组区段和另一个亲本的9个基因组区段的病毒颗粒。我们已经分离了两套完整的单抗，那些含有一个血清2型基因组片段和9个血清3型基因组片段，和那些含有1个血清3型基因组片段和9个血清1型基因组片段。我们已经使用前一组单重拮抗剂（因为呼肠孤病毒血清型2和3与血清型1和3的相关性较低）来评估所有10个基因组区段或它们编码的蛋白质在控制呼肠孤病毒增殖周期的参数（如产量大小、病毒ssRNA、dsRNA和蛋白质合成的程度、噬斑大小和致细胞病变性）中的作用。主要调查结果包括：蛋白质λ2、μ1/μ 1C和σ3控制RNA和蛋白质合成的产量大小和程度;蛋白质μ2和σ1控制细胞病变效应的严重程度;蛋白质σ1、μ1/μ 1C和μ2控制噬斑大小。鉴定单应答子表型可用于鉴定哪些病毒蛋白质在功能上参与呼肠孤病毒增殖周期的各个阶段。
When cultured cells are injected with mixtures of cores of two reovirus strains, a high proportion of reassortants are monoreassortants, that is, virus particles that contain one genome segment of 1 parent and 9 genome segments of the other. We have isolated two complete sets of monoreassortants, those that contain a single serotype 2 genome segment and 9 serotype 3 genome segments, and those that contain 1 serotype 3 genome segment and 9 serotype 1 genome segments. We have used the former set of monoreassortants (because reovirus serotypes 2 and 3 are less closely related than serotypes 1 and 3) to assess the effect of all 10 genome segments, or rather of the proteins that they encode, in controlling parameters of the reovirus multiplication cycle such as yield size, extent of viral ssRNA, dsRNA and protein synthesis, plaque size, and cytopathogenicity. Among the major findings are: proteins λ2, μ1/gm1 C, and σ3 control yield size and extent of RNA and protein synthesis; proteins μ2 and σ1 control severity of cytopathic effects; and proteins σ1, μ1/μ1 C, and μ2 control plaque size. Identification of monoreassortant phenotypes is useful for identifying which viral proteins are functionally involved at the various stages of the reovirus multiplication cycle.