Transgenic mice for Cre-inducible over expression of the oncogenes c-MYC and Pim-1 in multiple tissues
Transgenic mice for Cre-inducible over expression of the oncogenes c-MYC and Pim-1 in multiple tissues
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DOI：
10.1002/dvg.20235
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发表时间：
2006-10-01
期刊：
影响因子：
1.5
通讯作者：
Abdulkadir, Sarki A.
中科院分区：
文献类型：
--
作者：
Roh, Meejeon;Kim, Jongchan;Abdulkadir, Sarki A.
转录因子c-MYC和丝氨酸-苏氨酸激酶Pim-1在发育和癌症中具有多种作用，包括在淋巴瘤发生和前列腺肿瘤发生中。在一些癌症中，MYC和Pim-1癌基因共表达，并显示出显著的协同性。为了便于分析MYC和Pim-1在特定细胞类型和发育阶段中的病理作用，我们产生了携带Cre诱导的MYC/Pim-1转基因的小鼠。小鼠携带组成型表达的lacZ标记和沉默的MYC/Pim-1基因。Cre介导的重组导致lacZ标记的缺失和MYC/Pim-1转基因的同时激活。此外，Pim-1小鼠携带碱性磷酸酶基因作为重组的阳性标记。建立了每个基因的小鼠品系，其在多种组织中显示出不同的表达模式。通过与Cre转基因小鼠交配，确认所有品系的体内重组。这些小鼠为研究MYC和Pim-1在各种组织中过表达的意义提供了有价值的资源。
The transcription factor c-MYC and the serine-threonine kinase Pim-1 have multiple roles in development and cancer, including in lymphomagenesis and prostate tumorigenesis. In some cancers, MYC and Pim-1 oncogenes are co-expressed and show marked cooperativity. To facilitate the analysis of the pathological roles of MYC and Pim-1 in specific cell types,and developmental stages, we generated mice carrying Cre-inducible MYC/Pim-1 transgenes. The mice carry a constitutively expressed lacZ marker and silent MYC/Pim-1 genes. Cre-mediated recombination results in deletion of the lacZ marker and concurrent activation of the MYC/Pim-1 transgene. In addition, the Pim-1 mice harbor an alkaline phosphatase gene as a positive marker for recombination. Mouse lines for each gene were established, which show distinct patterns of expression in multiple tissues. In vivo recombination was confirmed for all lines by breeding to Cre transgenic mice. These mice provide a valuable resource for investigating the significance of MYC and Pim-1 overexpression in various tissues.