Photoelectric dye-coupled polyethylene film as a prototype of retinal prostheses
Photoelectric dye-coupled polyethylene film as a prototype of retinal prostheses
复制标题
DOI：
10.1111/j.1525-1594.2004.29010.x
复制
发表时间：
2005-01-01
影响因子：
2.4
通讯作者：
Suga, S
中科院分区：
文献类型：
--
作者：
Uji, A;Matsuo, T;Suga, S
光电染料吸收光并将光子能量转化为电势，以前已经证明可以刺激培养的视网膜神经元。在这项研究中，将一种光电染料偶联到聚乙烯薄膜表面，并在体外使用胚胎期12天的鸡胚胎视网膜组织进行测试，此时视网膜感光细胞的外段尚未发育。通过发烟硝酸将羧基部分引入到聚乙烯薄膜表面，然后通过酰胺键将光电染料2-[2-[4-（二丁基氨基）苯基]乙烯基]-3-羧甲基苯并噻唑溴偶联到薄膜上。与未与染料偶联聚乙烯膜接触的视网膜组织相比，用Fluo-4观察到放置在染料偶联聚乙烯膜上的视网膜组织的细胞内钙升高。这种反应可被钙环吡啶（一种电压门控钙通道阻滞剂）和细胞外钙消耗所抑制。光电染料偶联在聚乙烯薄膜表面，在解剖显微镜下吸收光并刺激视网膜组织中的神经元，表明该染料偶联膜可作为视网膜假体的原型。
Photoelectric dyes, which absorb light and convert photon energy to electric potentials, have been previously shown to stimulate retinal neurons in culture. In this study, a photoelectric dye was coupled to a polyethylene film surface and tested in vitro using retinal tissues from chick embryos at the 12-day embryonic stage, at which time outer segments of retinal photoreceptor cells have not yet developed. Carboxyl moieties were introduced to a polyethylene film surface by fuming nitric acid, and then a photoelectric dye, 2-[2-[4-(dibutylamino)phenyl]ethenyl]-3-carboxymethylbenzothiazolium bromide, was coupled to the film through amide linkage. Intracellular calcium elevation was observed with Fluo-4 in retinal tissues placed on the dye-coupled polyethylene film, in contrast to retinal tissues which had no contact with the film. The response was inhibited by calcicludine, a voltage-gated calcium channel blocker, and also by extracellular calcium depletion. The photoelectric dye, coupled to the polyethylene film surface, absorbed light under a dissecting microscope and stimulated neurons in retinal tissues, showing that the dye-coupled film could be used as a prototype of retinal prostheses.