Evaluating Adverse Effects of Inhaled Nanoparticles by Realistic In Vitro Technology.
Evaluating Adverse Effects of Inhaled Nanoparticles by Realistic In Vitro Technology.
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DOI：
10.3390/nano7020049
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发表时间：
2017-02-22
期刊：
影响因子：
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通讯作者：
Burtscher H
中科院分区：
文献类型：
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作者：
Geiser M;Jeannet N;Fierz M;Burtscher H
含有纳米颗粒(NP)的日常产品的数量正在迅速增加。粉末、分散体或喷雾剂中的NP对意外接触是一种未知的风险，特别是在NP生产和加工期间的工作场所，以及对于使用含NP喷雾剂的任何健康状况和年龄的消费者来说。我们开发了用于体外毒性的纳米气雾室(NACIVT)，这是一种便携式仪器，用于在体外对吸入NP进行真实的安全性测试，并评估了银(Ag)和碳(C)NP-这两种最广泛使用的纳米材料-对正常和受损的呼吸道上皮细胞的影响。我们回顾了NACIVT的发展，物理性能，以及对气液界面(ALI)细胞培养的短期和长期暴露的适用性，涉及到现实的吸入毒理学体外测试系统的先决条件，并与其他商业上可用的、特征良好的系统进行了比较。我们还回顾了应用于体外细胞培养的剂量，并承认单一暴露于实际剂量的火花产生的20纳米银或CNP会导致对这两种NP类型的小的、相似的细胞反应，并且细胞因子的释放通常随着NP剂量的增加而增加。
The number of daily products containing nanoparticles (NP) is rapidly increasing. NP in powders, dispersions, or sprays are a yet unknown risk for incidental exposure, especially at workplaces during NP production and processing, and for consumers of any health status and age using NP containing sprays. We developed the nano aerosol chamber for in vitro toxicity (NACIVT), a portable instrument for realistic safety testing of inhaled NP in vitro and evaluated effects of silver (Ag) and carbon (C) NP—which belong to the most widely used nanomaterials—on normal and compromised airway epithelia. We review the development, physical performance, and suitability of NACIVT for short and long-term exposures with air-liquid interface (ALI) cell cultures in regard to the prerequisites of a realistic in vitro test system for inhalation toxicology and in comparison to other commercially available, well characterized systems. We also review doses applied to cell cultures in vitro and acknowledge that a single exposure to realistic doses of spark generated 20-nm Ag- or CNP results in small, similar cellular responses to both NP types and that cytokine release generally increased with increasing NP dose.