Herbidospora solisilvae sp. nov., a novel cellulose-degrading actinobacterium isolated from forest soil
Herbidospora solisilvae sp. nov., a novel cellulose-degrading actinobacterium isolated from forest soil
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草芽孢属 solilvae sp.
DOI：
10.1007/s10482-021-01542-6
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发表时间：
2021-03-01
影响因子：
2.6
通讯作者：
Xiang, Wensheng
中科院分区：
文献类型：
--
作者：
Yu, Mingying;Zhou, Rui;Xiang, Wensheng
从海南省三亚市林春岭森林公园土壤中分离到一株革兰氏染色阳性、纤维素降解放线菌，命名为NEAU-GS 84 T。形态学和化学分类学特征表明，菌株NEAU-GS 84 T属于草孢菌属（Herbidospora）。16 S rRNA基因序列分析证实菌株NEAU-GS84 T属于草孢属，与依兰草孢菌（Herbidospora yilanensis）JCM 18062 T（16 S rRNA基因序列相似性为99.2%）和球孢草孢菌（Herbidospora galbida）NEAU-GS14 T（99.0%）的亲缘关系最近。菌株NEAU-GS 84 T的胞壁主要含有内消旋二氨基庚二酸，全细胞水解产物主要含有葡萄糖、马度糖和核糖。主要极性脂质为二磷脂酰甘油、磷脂酰甲基乙醇胺、磷脂酰乙醇胺、羟基磷脂酰乙醇胺、磷酸糖脂、两种磷脂酰肌醇甘露糖苷和一种未鉴定的磷脂。主要的甲基萘醌是MK-10（H4）。主要脂肪酸为10-甲基C17：0、C17：0和异C16：0。这些化学分类学数据证实了菌株NEAU-GS 84 T隶属于Herbidospora。DNA G+C含量为70.7mol%。该菌株基因组大小约为8.37 Mb，含有41个纤维素结合域合成基因、13个β-葡萄糖苷酶合成基因、6个内切葡聚糖酶合成基因和9个木聚糖酶合成基因。根据DNA数字杂交和平均核苷酸同一性值，新菌株NEAU-GS 84 T可以与其最近的亲戚区分开来。因此，该菌株代表了草孢属的一个新物种，建议将其命名为Herbidospora solisilarum sp. nov.。模式菌株为NEAU-GS 84 T（= CCTCC AA 2018041 T = JCM 33460 T）。
An aerobic, non-motile, Gram-stain positive, cellulose-degrading actinobacterium, designated strain NEAU-GS84T, was isolated from a forest soil sample collected from Linchun Ridge Forest Park in Sanya, Hainan Province, China, and characterized using a polyphasic approach. Morphological and chemotaxonomic characteristics indicated that strain NEAU-GS84Tcould belong to the genus Herbidospora. The 16S rRNA gene sequence analysis indeed confirmed that strain NEAU-GS84Tbelonged to the genus Herbidospora and was most closely related to Herbidospora yilanensis JCM 18062T(99.2% 16S rRNA gene sequence similarity) and Herbidospora galbida NEAU-GS14T(99.0%). The cell wall of strain NEAU-GS84Tcontained meso-diaminopimelic acid as the major diamino acid and the whole-cell hydrolysates mainly contained glucose, madurose and ribose. The major polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyphosphatidylethanolamin, phosphoglycolipids, two phosphatidylinositol mannosides and one unidentified phospholipid. The predominant menaquinone was MK-10(H4). Major fatty acids were 10-methly C17:0, C17:0and iso-C16:0. These chemotaxonomic data substantiated the affiliation of strain NEAU-GS84Tto the genus Herbidospora. The DNA G+C content was 70.7 mol%. The genome size of strain NEAU-GS84Tis about 8.37 Mb and contained 41 cellulose-binding domain synthesis genes, 13 β-glucosidase synthesis genes, 6 endoglucanase synthesis genes and 9 xylanase synthesis genes. Based on digital DNA-DNA hybridization and average nucleotide identity values, the new strain NEAU-GS84Tcould be differentiated from its closest relatives. Therefore, the strain represents a novel species of the genus Herbidospora, for which the name Herbidospora solisilvae sp. nov. is proposed. The type strain is NEAU-GS84T(= CCTCC AA 2018041T= JCM 33460T).