Determination of hexitols by reversed phase liquid chromatography using on-line complexation with molybdate ion
Determination of hexitols by reversed phase liquid chromatography using on-line complexation with molybdate ion
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钼酸根离子在线络合反相液相色谱法测定己糖醇
DOI：
10.1016/j.aca.2016.12.014
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发表时间：
2017
影响因子：
6.2
通讯作者：
Kazuichi Hayakawa
中科院分区：
文献类型：
--
作者：
Tomoko Kemmei;Shuji Kodama;Atsushi Yamamoto;Yoshinori Inoue;Kazuichi Hayakawa
提出了一种用反相液相色谱-紫外检测法同时测定三种己糖醇（甘露醇、山梨醇和卫矛醇）的新方法。当向移动的相中加入异戊二醇时，它与己糖醇形成复合物，该复合物可在反相C30柱上分离，并通过247 nm处的UV吸收检测。移动的流动相（pH 3.1）由0.1 mM碳酸二钠和1 mM磷酸组成。其他糖醇，如木糖醇和木糖醇，和葡萄糖不能在这些条件下检测。在S/N = 10下计算的检查的三种己糖醇的定量限为0.001 mM，检测器响应在0.001-0.3 mM范围内呈线性。该方法成功地测量了糖果样品中的己糖醇，所提出的方法得到的结果与酶法得到的结果一致。
A new approach is proposed to determine three hexitols (mannitol, sorbitol and dulcitol) by reversed phase liquid chromatography and ultraviolet detection. When molybdate ion is added to the mobile phase, it forms complexes with hexitols that can be separated on a reversed phase C30 column and detected by UV absorption at 247 nm. The mobile phase (pH 3.1) consisted of 0.1 mM disodium molybdate and 1 mM phosphoric acid. Other sugar alcohols, such as erythritol and xylitol, and glucose could not be detected under these conditions. The quantification limits of the examined three hexitols calculated at S/N = 10 were 0.001 mM and the detector response was linear in the range 0.001–0.3 mM. The method successfully measured these hexitols in candy samples, and the results obtained by the proposed method agreed well with those obtained by enzymatic methods.