MORPHOGENESIS OF POLIOVIRUS .2. DEMONSTRATION OF A NEW INTERMEDIATE, PROVIRION
MORPHOGENESIS OF POLIOVIRUS .2. DEMONSTRATION OF A NEW INTERMEDIATE, PROVIRION
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DOI：
10.1128/jvi.12.5.1122-1130.1973
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发表时间：
1973-01-01
影响因子：
5.4
通讯作者：
BALTIMORE, D
中科院分区：
文献类型：
--
作者：
FERNANDEZTOMAS, CB;BALTIMORE, D
脊髓灰质炎病毒感染的细胞含有一种以前未被认识的颗粒，它似乎是病毒体合成的中间体，因此被命名为前病毒。它在约125 S处沉积，含有三种前衣壳蛋白VP-0、VP-1和VP-3，并具有35 S病毒RNA。它被十二烷基硫酸钠和EDTA破坏，但RNA抵抗核糖核酸酶的消化。脉冲相位实验和使用病毒特异性抑制剂胍的研究都表明，前病毒是由新产生的RNA与原衣壳结合形成的。VP-0裂解形成VP-2和VP-4是在前病毒粒子形成之后，并且是脊髓灰质炎病毒形态发生的最后一步。
Poliovirus-infected cells contain a previously unrecognized particle which appears to be an intermediate in virion synthesis and therefore has been named proviron. It sediments at about 125S, contains the three procapsid proteins, VP-0, VP-1, and VP-3, and has 35Sviral RNA. It is disrupted both by sodium dodecyl sulfate and EDTA but the RNA resists digestion by ribonuclease. Pulsechase experiments and studies employing the virus-specific inhibitor, guanidine, all indicate that the proviron is formed by combination of newly made RNA with the procapsid. Cleavage of VP-0 to form VP-2 and VP-4 follows formation of the provirion and would be the final step in poliovirus morphogenesis.