Retrospective molecular integrated epidemiology of Chagas disease in Colombia
Retrospective molecular integrated epidemiology of Chagas disease in Colombia
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DOI：
10.1016/j.meegid.2013.08.028
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发表时间：
2013-12-01
影响因子：
3.2
通讯作者：
David Ramirez, Juan
中科院分区：
文献类型：
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作者：
Guhl, Felipe;David Ramirez, Juan
美洲锥虫病是一种非常复杂的人畜共患病，在整个南美洲、中美洲和墨西哥都存在，并继续对该区域各国的健康构成严重威胁。寄生虫感染150种来自24个家庭的家庭和野生哺乳动物，并显示出显着的遗传变异性，证明在至少7个离散的分型单位（DTU的）命名为TcI-TcVI与存在一个新的基因型与蝙蝠命名为TcBat。这些DTU显示了广泛的地理和宿主分布。我们的研究旨在建立克氏锥虫的遗传多样性和感染患者的不同临床表现之间的关系，并解开在哥伦比亚的流行病和地方病的情况下的分子生态流行病学。我们在哥伦比亚的17个部门进行了密集的采样，其中包括11种锥蝽，9种哺乳动物水库物种和人类，并获得了637个生物克隆，随后使用核和线粒体分子标记进行了分析。TcI最常见（80.7%），其次是TcII（7.2%）、TcIII（3.9%）、TcIV（5%）、TcV（0.8%）、TcVI（1.6%）和TcBat（0.8%）。在国内疫源地内，TcI（70%）最常见，其次是TcII（20%），TcIII（20%），TcIV（3.6%）、TcV（2.2%）和TcVI（2.6%）;在森林样病灶中，TcI（85%）最常见，其次是TcII（0.3%）、TcIII（5.5%）、TcIV（7%）、TcVI（1.1%）和TcBat（1.1%）。结果表明，发生的七个DTU和严格的协会独立DTU的主机和环境在哥伦比亚。本文讨论了这些影响。(C)2013爱思唯尔有限公司版权所有。
American trypanosomiasis is a very complex zoonosis that is present throughout South America, Central America, and Mexico and continues to represent a serious threat to the health of countries in the region. The parasite infects 150 species from 24 families of domestic and wild mammals and shows remarkable genetic variability evinced in at least seven discrete typing units (DTU's) named TcI-TcVI with the presence of a novel genotype associated with bats named TcBat. These DTUs show a wide range of geographical and host distributions. Our study aimed to establish the relationship between the genetic diversity of Trypanosoma cruzi and the diverse clinical manifestations of infected patients and unravel the molecular eco-epidemiology in the epizootic and enzootic scenarios in Colombia. We undertook intensive sampling in 17 departments of Colombia among 11 triatomine species, 9 mammalian reservoir species and humans and obtained 637 biological clones that were subsequently analyzed using nuclear and mitochondrial molecular markers. TcI was the most prevalent (80.7%) followed by TcII (7.2%), TcIII (3.9%), TcIV (5%), TcV (0.8%), TcVI (1.6%) and TcBat (0.8%). Within domestic foci, TcI (70%) was the most prevalent, followed by TcII (20%), TcIII (1.6%), TcIV (3.6%), TcV (2.2%) and TcVI (2,6%); within sylvatic foci, TcI (85%) was the most prevalent, followed by TcII (0.3%), TcIII (5.5%), TcIV (7%), TcVI (1.1%) and TcBat (1.1%). The results suggest the occurrence of the seven DTUs and strict associations of independent DTUs with the host and environment in Colombia. The implications are discussed herein. (C) 2013 Elsevier B.V. All rights reserved.