An in vitro system for accurate transcription initiation of chloroplast protein genes.
An in vitro system for accurate transcription initiation of chloroplast protein genes.
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用于叶绿体蛋白基因精确转录起始的体外系统。
DOI：
10.1093/nar/13.4.1283
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发表时间：
1985
影响因子：
14.9
通讯作者：
Chua,NH
中科院分区：
文献类型：
--
作者：
OrozcoJr,EM;Mullet,JE;Chua,NH
我们已经从菠菜叶绿体中建立了一个同源的体外系统，该系统可以正确启动质体核酮糖-1，5-二磷酸羧化酶大亚基（rbcL）和质体ATP酶β亚基（atpB）基因的转录。RNA聚合酶活性对利福平不敏感。体外转录的程度是提取物：模板比例的函数。体外转录效率为每100个模板每小时1个转录本以上。
We have developed an homologousin vitrosystem from spinach chloroplasts that correctly initiates transcription of the plastid genes for the large subunit of ribulose-1,5-bisphosphate carboxylase (rbcL) and the β subunit of the plastid ATPase (atpB) The transcriptionally active extracts from spinach chloroplasts require circular DNA templates for specific initiation. The RNA polymerase activity is insensitive to rifampicin. The extent of transcriptionin vitrois a function of the extract:template ratio. The efficiency of therbcL transcriptionin vitrois more than one transcript per one hundred templates per hour.