Compressive mechanical stress promotes osteoclast formation through RANKL expression on synovial cells
Compressive mechanical stress promotes osteoclast formation through RANKL expression on synovial cells
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DOI：
10.1016/j.tripleo.2006.05.026
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发表时间：
2007-03-01
期刊：
影响因子：
--
通讯作者：
Nishihara, Tatsuji
中科院分区：
文献类型：
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作者：
Ichimiya, Hisashi;Takahashi, Tetsu;Nishihara, Tatsuji
目标。我们研究了压缩机械应力对滑膜细胞破骨细胞生成的影响，以阐明颞下颌关节 (TMJ) 疾病中这些细胞形成破骨细胞的机制。研究设计。从大鼠膝关节中分离滑膜细胞，并使用常规方法连续压缩。通过逆转录聚合酶链反应、免疫印迹和免疫荧光染色分析滑膜细胞中受体激活剂核因子κB配体（RANKL）mRNA和蛋白的表达。小鼠骨髓细胞与滑膜细胞培养7天，检测破骨细胞。结果。滑膜细胞中RANKL mRNA和蛋白的表达随着压力的增加而增加。当小鼠骨髓细胞与持续压缩的滑膜细胞一起培养时，形成了抗酒石酸酸性磷酸酶阳性的多核细胞。骨保护素完全抑制压缩滑膜细胞培养诱导的破骨细胞形成。结论。我们的结果表明，受压滑膜细胞中 RANKL 的表达增强了破骨细胞的形成，而持续的压力可能会诱导 TMJ 中破骨细胞的骨破坏。
Objectives. We investigated the effects of compressive mechanical stress on osteoclastogenesis of synovial cells to clarify the mechanism of osteoclast formation by those cells in temporomandibular joint (TMJ) disorders.Study design. Synovial cells were isolated from rat knee joints and continuously compressed using a conventional method. The expression of receptor activator nuclear factor kappa B ligand (RANKL) mRNA and protein in synovial cells was analyzed by reverse transcriptase-polymerase chain reaction, immunoblotting, and immunofluorescence staining. Mouse bone marrow cells were cultured with synovial cells for 7 days to detect osteoclasts.Results. The expressions of RANKL mRNA and protein in synovial cells were increased with compressive force. When mouse bone marrow cells were cultured with continuously compressed synovial cells, tartrate-resistant acid phosphatase-positive multinucleated cells were formed. Osteoprotegerin completely inhibited osteoclast formation induced by culturing with compressed synovial cells.Conclusion. Our results indicated that the expression of RANKL in compressed synovial cells enhanced osteoclast formation, whereas continuous compressive force may induce osteoclastic bone destruction in the TMJ.