Anti-osteoporosis effects of 1,4-dihydroxy-2-naphthoic acid in ovariectomized mice with increasing of bone density
Anti-osteoporosis effects of 1,4-dihydroxy-2-naphthoic acid in ovariectomized mice with increasing of bone density
复制标题
DOI：
10.1016/j.ajoms.2015.07.001
复制
发表时间：
2016-01-01
影响因子：
0.4
通讯作者：
Iida, Seiji
中科院分区：
文献类型：
--
作者：
Kita, Kenichiro;Yamachika, Eiki;Iida, Seiji
目的：1，4-二羟基-2-萘甲酸（DHNA）是费氏丙酸杆菌ET-3发酵后代谢产物的主要成分。在这项研究中，DHNA，维生素K2（VK 2），和利塞膦酸钠（Ris）给予卵巢切除（OVX）小鼠，以确定其抑制骨量减少的作用。每天给药8周。随后，从眼眶静脉丛采集血液。切除双侧股骨和L5腰椎。为了确定药物的作用，我们通过micro-CT对左股骨和L5进行了组织形态计量学分析，对血清样本进行了生化分析，并对右股骨进行了组织学分析。总骨矿物质密度、骨体积密度、骨小梁厚度、骨小梁数量、骨小梁分离，组织学分析显示DHNA和Ris组骨吸收较OVX组明显改善。此外，生化分析表明，生产的炎症细胞因子被抑制在DHNA和Ris groups.Conclusions：目前的研究结果表明，DHNA抑制骨质疏松症小鼠的骨量减少，这表明它可能是一种替代治疗绝经后骨质疏松症。(C)2015年亚洲AOMS、ASOMP、JSOP、JSOMS、JSOM和JAMI。由爱思唯尔有限公司出版。保留所有权利。（星星）
Objective: 1,4-Dihydroxy-2-naphthoic acid (DHNA) is the main component of the metabolic products after fermentation by Propionibacterium freudenreichii ET-3. In this study, DHNA, vitamin K2 (VK2), and risedronate (Ris) were administered to ovariectomized (OVX) mice to ascertain their suppressive effects on bone mass reduction.Methods: Fifty mice were divided into five groups: Sham, OVX, DHNA, VK2, and Ris groups. The drugs were administered daily for 8 weeks. Subsequently, blood was collected from the orbital venous plexus. The bilateral femurs and L5 lumbar vertebra were excised. To determine the effects of the drugs, we performed histomorphometric analyses of the left femur and L5 by micro-CT, biochemical analyses of serum samples, and histological analyses of the right femur.Results: The results for the total bone mineral density, bone volume density, trabecular thickness, trabecular number, trabecular separation, and histological analyses showed that bone resorption was improved in the DHNA and Ris groups compared with the OVX group. Furthermore, the biochemical analyses revealed that the production of inflammatory cytokines was suppressed in the DHNA and Ris groups.Conclusions: The present findings show that DHNA suppresses the bone mass reduction in OVX mice, suggesting that it could be an alternative treatment for postmenopausal osteoporosis. (C) 2015 Asian AOMS, ASOMP, JSOP, JSOMS, JSOM, and JAMI. Published by Elsevier Ltd. All rights reserved.(star)