CHL1 promotes insulin secretion and negatively regulates the proliferation of pancreatic β cells
CHL1 promotes insulin secretion and negatively regulates the proliferation of pancreatic β cells
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DOI：
10.1016/j.bbrc.2020.03.040
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发表时间：
2020-05-14
影响因子：
3.1
通讯作者：
Yang, Tao
中科院分区：
文献类型：
--
作者：
Jiang, Hemin;Liu, Yuwei;Yang, Tao
Cell adhesion molecule L1-like protein (CHL1) is a member of neural recognition molecules of immunoglobulin superfamily primarily expressing in the nervous system. CHL1 regulates neuronal migration, axonal growth, and dendritic projection. Downregulation of CHL1 has been reported in beta cells of patients with type 2 diabetes (T2DM). However, the detailed role of CHL1 in b cells has not been characterized. In this study, Real-Time PCR and Western blot were applied to investigate the tissue/cell distribution and expression of CHL1. Gain- or loss-of function studies were conducted in MIN6 cells to determine the effects of CHL1 on cell proliferation, apoptosis, cell cycle, and insulin secretion. Following silencing of CHL1 in MIN6 cells (si-CHL1), insulin secretion and the number of insulin secretary granules
