Neisseria meningitidis lactate permease is required for nasopharyngeal colonization
Neisseria meningitidis lactate permease is required for nasopharyngeal colonization
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DOI：
10.1128/iai.73.9.5762-5766.2005
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发表时间：
2005-09-01
影响因子：
3.1
通讯作者：
Tang, CM
中科院分区：
文献类型：
--
作者：
Exley, RM;Goodwin, L;Tang, CM
脑膜炎奈瑟氏菌是一种人类特异性病原体，是正常鼻咽植物群的一部分。关于脑膜炎球菌在上呼吸道定植期间对存活的代谢限制知之甚少。在这里，我们表明，葡萄糖和乳酸，脑膜炎球菌生长的碳能源，存在于毫摩尔浓度在鼻咽组织。我们使用乳酸摄入缺陷的突变体（C311 Delta lctP）来研究这种能量来源在定植过程中的贡献。鼻咽组织的外植体接种野生型菌株（C311）和C311 Delta lctP;突变体在18小时后恢复到显著低于C311的水平（P = 0.01）。这种缺陷不是由于粘附素表达的变化或C311 Delta lctP对上皮细胞的初始粘附。相反，乳酸盐似乎是细菌在定植期间的重要能量来源，并且是细菌在鼻咽组织中生长所必需的。对其他不能吸收特定营养物质的菌株的研究应该提供有关N。脑膜炎在分娩期间持续存在。
Neisseria meningitidis is a human specific pathogen that is part of the normal nasopharyngeal flora. Little is known about the metabolic constraints on survival of the meningococcus during colonization of the upper airways. Here we show that glucose and lactate, both carbon energy sources for meningococcal growth, are present in millimolar concentrations within nasopharyngeal tissue. We used a mutant defective for the uptake of lactate (C311 Delta lctP) to investigate the contribution of this energy source during colonization. Explants of nasopharyngeal tissue were inoculated with the wild-type strain (C311) and C311 Delta lctP; the mutant was recovered at significantly lower levels (P = 0.01) than C311 18 h later. This defect was not due to changes in the expression of adhesins or initial adhesion in C311 Delta lctP to epithelial cells. Instead, lactate appears to be important energy source for the bacterium during colonization and is necessary for growth of the bacterium in nasopharyngeal tissue. Studies with other strains defective for the uptake of specific nutrients should provide valuable information about the environment in which N. meningitidis persists during carriage.