Comparative analysis of the alphavirus-based vectors expressing Rift Valley fever virus glycoproteins
Comparative analysis of the alphavirus-based vectors expressing Rift Valley fever virus glycoproteins
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DOI：
10.1016/j.virol.2007.04.014
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发表时间：
2007-09-15
期刊：
影响因子：
3.7
通讯作者：
Frolov, Ilya
中科院分区：
文献类型：
--
作者：
Gorchakov, Rodion;Volkova, Eugenia;Frolov, Ilya
在过去的十年中，甲病毒被广泛用于表达异源遗传信息和开发针对各种人类和动物病原体的重组疫苗。在这项研究中，我们比较了一些载体的基因组的辛德毕斯（SINV）和委内瑞拉马脑炎（VEEV）病毒的表达裂谷热病毒（RVFV）包膜糖蛋白Gn和诱导保护性免疫反应对RVFV感染的能力。我们的结果表明，（i）与基于SINV基因组设计的类似系统相比，基于VEEV的表达系统的应用似乎是有利的。(ii)甲病毒特异性E3和E2蛋白和弗林蛋白酶特异性切割位点可用于工程化分泌形式的蛋白。(iii)甲病毒可以被修饰用于在嵌合的感染性病毒颗粒的表面上表达异源蛋白的大片段。因此，基于甲病毒的表达系统可能具有超越其当前用作复制子或双亚基因组载体的更广泛应用的潜力。(c)2007年爱思唯尔公司All rights reserved.
During the last decade, alphaviruses became widely used for expression of heterologous genetic information and development of recombinant vaccines against a variety of human and animal pathogens. In this study, we compared a number of vectors based on the genome of Sindbis (SINV) and Venezuelan equine encephalitis (VEEV) viruses for their ability to express the Rift Valley fever virus (RVFV) envelope glycoprotein Gn and induce a protective immune response against RVFV infection. Our results suggest that (i) application of VEEV-based expression systems appears to be advantageous, when compared to similar systems designed on the basis of the SINV genome. (ii) Alphavirus-specific E3 and E2 proteins and furin-specific cleavage sites can be used for engineering secreted forms of the proteins. (iii) Alphaviruses can be modified for expression of the large fragments of heterologous proteins on the surface of chimeric, infectious viral particles. Thus, alphavirus-based expression systems may have the potential for a broader application beyond their current use as replicons or double-subgenomic vectors. (c) 2007 Elsevier Inc. All rights reserved.