Sequence and organization of the complete mitochondrial genome of the Chinese three-keeled pond turtle Chinemys reevesi
Sequence and organization of the complete mitochondrial genome of the Chinese three-keeled pond turtle Chinemys reevesi
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发表时间：
2005
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通讯作者：
Pu You
中科院分区：
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作者：
Pu You
The phylogenic relationship of turtles to other vertebrate groups remains to be open. In order to provide molecular evidence to this issue, we analyzed the complete mitochondrial (mt) genome of the turtle Chinemys reevesi. DNA sequencing was based on PCR fragments from 16 primer pairs that were designed on the basis of mtDNA sequences of three related turtle species. The genome is 16 576 base pairs in length, contains 37 genes (two ribosomal RNA, 22 transfer RNA and 13 protein-coding genes) and a putative control region, a situation that is similar to that found in other vertebrates. The gene order is identical to that of the four other turtle species so far reported. Those genes are arranged compactly with no introns and few intergenic nucleotides. Twenty-two tRNA genes include 2 tRNA Ser, 2 tRNA Leu and 18 different species of other tRNA genes each. The tRNA Gln, tRNA Ala, tRNA Asn, tRNA Cys ,tRNA Tyr, tRNA Ser(the second one) ,tRNA Glu and tRNA Pro genes are encoded on the light strand, and the remainder is on the heavy strand. The length of the tRNA genes ranges from 66 bp (tRNA Ser and L-strand) to 77 bp (tRNA Ttp). All protein-coding genes begin with ATG as initiation codons except CO1 that uses GTG. Most protein-coding genes, including CO2, ATP8, CO3, ND4L, ND4, ND5 and Cyt b, have a TAA termination codon, while the others have incomplete termination codons. Among the 13 protein-coding genes, only ND6 is located on the light strand, the others are on the heavy strand. The order of the 13 protein-coding genes is the same as in mammals, amphibians and crocodiles. The total length of the 13 protein-coding genes makes up 11 378 bp,which is 68.64% of the mt genome. The 12S rRNA gene of Chinemys reevesi begins at 73 bp and ends at 1 041 bp. The 16S rRNA gene begins at 1 112 bp and ends at 2 715 bp. The control region of the Chinemys reevesi mt genome is 1 072 bp long, which is 68.66% AT-rich region, and its flanking tRNA genes are 5′-tRNA Pro and 3′-tRNA Phe. The sequence was submitted to GenBank and got a GenBank accession number: AY676201.
