In Vivo study of ligament-bone healing after anterior cruciate ligament reconstruction using autologous tendons with mesenchymal stem cells affinity peptide conjugated electrospun nanofibrous scaffold
In Vivo study of ligament-bone healing after anterior cruciate ligament reconstruction using autologous tendons with mesenchymal stem cells affinity peptide conjugated electrospun nanofibrous scaffold
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DOI：
10.1155/2013/831873
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发表时间：
2013
影响因子：
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通讯作者：
Jing-xian Zhu;Xin Zhang;Zhenxing Shao;Linghui Dai;La Li;Xiaoqing Hu;Xiaokun Wang;Chun-yan Zhou;Y. Ao
中科院分区：
文献类型：
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作者：
Jing-xian Zhu;Xin Zhang;Zhenxing Shao;Linghui Dai;La Li;Xiaoqing Hu;Xiaokun Wang;Chun-yan Zhou;Y. Ao
静电纺丝纳米纤维支架是近年来广泛应用于组织再生的材料。据报道，具有特定拓扑特征的纳米纤维能够诱导MSC的成骨分化。在这项体内研究中，在自体肌腱两端包裹网格状纳米纤维支架的自体肌腱移植物用于促进兔ACL重建后韧带骨愈合的早期阶段。为了利用来自骨髓的天然MSC，将MSC特异性亲和肽E7缀合至纳米纤维网。3个月后，H-E评估和I型、II型和III型胶原蛋白的特异性染色显示生物活性支架重建组中具有典型的四个区域（骨、钙化纤维软骨、纤维软骨和韧带）的直接韧带-骨插入。纳米纤维支架结合E7肽组骨隧道直径小于对照组。替代复合物的失效载荷也表明使用生物活性支架的韧带-骨插入愈合更强。因此，具有特异性MSCs亲和肽的纳米纤维网格支架在促进ACL重建后韧带-骨愈合的早期阶段具有巨大的潜力。
Electrospinning nanofibrous scaffold was commonly used in tissue regeneration recently. Nanofibers with specific topological characteristics were reported to be able to induce osteogenic differentiation of MSCs. In this in vivo study, autologous tendon grafts with lattice-like nanofibrous scaffold wrapping at two ends of autologous tendon were used to promote early stage of ligamentbone healing after rabbit ACL reconstruction. To utilize native MSCs from bone marrow, an MSCs specific affinity peptide E7 was conjugated to nanofibrous meshes. After 3 months, H-E assessment and specific staining of collagen type I, II, and III showed direct ligament-bone insertion with typical four zones (bone, calcified fibrocartilage, fibrocartilage, and ligament) in bioactive scaffold reconstruction group. Diameters of bone tunnel were smaller in nanofibrous scaffold conjugated E7 peptide group than those in control group. The failure load of substitution complex also indicated a stronger ligament-bone insertion healing using bioactive scaffold. In conclusion, lattice-like nanofibrous scaffold with specific MSCs affinity peptide has great potential in promoting early stage of ligament-bone healing after ACL reconstruction.