Expression of the FAM5C in tongue squamous cell carcinoma
Expression of the FAM5C in tongue squamous cell carcinoma
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DOI：
10.3892/or_00000528
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发表时间：
2009-11-01
期刊：
影响因子：
4.2
通讯作者：
Shibahara, Takahiko
中科院分区：
文献类型：
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作者：
Kuroiwa, Tsukasa;Yamamoto, Nobuharu;Shibahara, Takahiko
本研究的目的是使用 Affymetrix 10K SNP 定位阵列对舌鳞状细胞癌 (SCC) 杂合性缺失 (LOH) 进行全基因组分析。在通过全基因组分析鉴定出的LOH基因中，我们分析了等位基因的不平衡性，以确定该基因的作用。我们使用该芯片对 5 例舌 SCC 样本中的 LOH 进行了全基因组分析。在通过全基因组分析鉴定出LOH的染色体区域中，我们利用微卫星标记再次确认了30例LOH的存在。通过实时定量 RTPCR 分析检测 15 例病例和 5 个舌 SCC 衍生细胞系中该区域 mRNA 的表达水平。 1q31.1 区域的 5 例病例均观察到 LOH。 1q31.1 区域仅存在 3 个微卫星标记（D1S1189、D1S2151 和 D1S2595）。在 18/30 (60%) 的 D IS 1189 位点、16/30 (53%) 的 D1S2151 位点和 21/30 (70%) 的 D1S2595 位点发现高频率的 LOH。仅序列相似性为5的家族成员C（FAM5C）基因位于1q31.1区域。舌鳞状细胞癌与正常组织中FAM5C mRNA表达水平差异有统计学意义。与正常口腔角质形成细胞 (NOK) 相比，所有舌 SCC 衍生细胞系均降低了 FAM5C mRNA 表达。我们得出结论，FAM5C 可能是舌 SCC 中的一种新型抑癌基因 (TSG)。
The purpose of this study was to perform a whole-genome analysis of loss of heterozygosity (LOH) in tongue squamous cell carcinoma (SCC) using the Affymetrix 10K SNP Mapping Array. In the gene which had been identified by whole-genome analysis of LOH, we analyzed allelic imbalance to identify the role of the gene. We applied whole-genome analysis of LOH in the specimens from the 5 cases of tongue SCC using this array. In the chromosomal region which had been identified by whole-genome analysis of LOH, we reconfirmed the existence of LOH in 30 cases using microsatellite markers. The expression levels of the mRNA in the region were examined in 15 cases and in 5 tongue SCC-derived cell lines by real-time quantitative RTPCR analysis. LOH was observed in all of the 5 cases in the 1q31.1 region. Only 3 microsatellite markers (D1S1189, D1S2151, and D1S2595) existed in the 1q31.1 region. A high frequency of LOH was found at the D IS 1189 locus in 18/30 (60%), D1S2151 locus in 16/30 (53%) and D1S2595 locus in 21/30 (70%). Only the Family with sequence similarity 5, member C (FAM5C) gene was located in the 1q31.1 region. There was statistically significant difference in the FAM5C mRNA expression levels between tongue SCC and normal tissues. All tongue SCC-derived cell lines decreased FAM5C mRNA expression compared with normal oral keratinocytes (NOKs). We conclude that FAM5C may be a novel tumor suppressor gene (TSG) in tongue SCC.