Sustained and Prolonged Delivery of Protein Therapeutics from Two-Dimensional Nanosilicates
Sustained and Prolonged Delivery of Protein Therapeutics from Two-Dimensional Nanosilicates
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DOI：
10.1021/acsami.8b17733
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发表时间：
2019-02-20
影响因子：
9.5
通讯作者：
Gaharwar, Akhilesh K.
中科院分区：
文献类型：
--
作者：
Cross, Lauren M.;Carrow, James K.;Gaharwar, Akhilesh K.
我们提出了一种纳米工程系统，用于持续和延长蛋白质治疗剂的递送，这有可能影响当前的骨科再生策略。具体来说，我们引入了具有高表面积和带电特性的二维纳米硅酸盐，用于递送活性蛋白质超过30天。纳米硅酸盐显示出高的结合效力，而不改变蛋白质构象和生物活性。释放的蛋白质能够保持高活性，如通过与外源性对照相比在低10倍的浓度下增强人间充质干细胞的分化所证明的。利用纳米硅酸盐作为递送载体可以最小化由于使用超生理剂量的蛋白质治疗剂用于矫形再生策略而观察到的负面副作用。
We present a nanoengineered system for sustained and prolonged delivery of protein therapeutics, which has the potential to impact current orthopedic regeneration strategies. Specifically, we introduce two-dimensional nanosilicates with a high surface area and charged characteristics for delivery of active proteins for more than 30 days. The nanosilicates show high binding efficacy without altering the protein conformation and bioactivity. The released proteins are able to maintain high activity as demonstrated by enhanced differentiation of human mesenchymal stem cells at 10-fold lower concentration compared to the exogenous control. Utilizing the nanosilicates as a delivery vehicle could minimize the negative side effects observed because of the use of supraphysiological dosages of protein therapeutics for orthopedic regeneration strategies.