Effects of dietary n-6/n-3 polyunsaturated fatty acid ratios on themass, and histological and ultrastructures of liver, spleen and thymus of 70-day-old Yangzhou Goslings
Effects of dietary n-6/n-3 polyunsaturated fatty acid ratios on themass, and histological and ultrastructures of liver, spleen and thymus of 70-day-old Yangzhou Goslings
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DOI：
10.1111/jpn.12371
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发表时间：
2016-04-01
影响因子：
2.7
通讯作者：
Zhang, J.
中科院分区：
文献类型：
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作者：
Wang, M. Z.;Ding, L. Y.;Zhang, J.
本试验旨在研究饲粮n-6/n-3多不饱和脂肪酸（PUFA）比例对70日龄扬州雏鹅脏器指数、肝脏、脾脏和胸腺组织学和超微结构的影响。选用21日龄扬州雏鹅160只，随机分为4组，分别饲喂n-6/n-3多不饱和脂肪酸（n-6/n-3 PUFA）为3：1 ~ 12：1的4种日粮。驯化1周后，进行为期6周的饲养试验。试验期结束后，屠宰雏鹅，取肝、脾、胸腺称重，进行组织学和超微结构观察。结果表明，3：1组的脏器指数显著高于其他3组，而线粒体面积在4组间无显著差异。低n-6/n-3 PUFA比（3：1和6：1）的日粮对肝脏、脾脏和胸腺的组织学和超微结构无影响。而在n-6/n-3 PUFA比例较高（9：1和12：1）的饲料中，核染色质被浓缩并边缘化，细胞膜向内收缩并破裂，线粒体膜受到一定程度的损伤。因此，高n-3 PUFA含量的日粮可促进雏鹅生长，维持肝、脾、胸腺等器官的细胞结构，从而提高雏鹅的免疫力。
The objective of this study was to determine the effects of dietary n-6/n-3 polyunsaturated fatty acid (PUFA) ratios on the organ indexes, and histological and ultrastructures of organs including liver, spleen and thymus in 70-day-old Yangzhou goslings. One-hundred and sixty 21-day-old Yangzhou goslings were randomly divided into 4 groups and fed 4 diets varying in the n-6/n-3 PUFA ratio from 3:1 up to 12:1. After 1-week acclimation, the feeding experiment lasted for 6weeks. At the end of the experimental period, goslings were slaughtered and the liver, spleen and thymus were weighed, and their histological and ultrastructures were examined. The results showed that the organ indices in the 3:1 group were remarkably higher than in the other three groups, whereas the mitochondrial square did not differ among four groups. The histological and ultrastructures of the liver, spleen and thymus were not affected by the diets with the lower n-6/n-3 PUFA ratios (3:1 and 6:1). However, feeding diets with the higher n-6/n-3 PUFA ratios (9:1 and 12:1), the nuclear chromatin was concentrated and marginalized; the cell membrane was contracted inwardly and disrupted; the mitochondrial membrane was damaged to some degree. In conclusion, the diet containing higher content of n-3 PUFA might improve immune capacity of goslings the animal by accelerating the growth and maintaining cellular structures of organs like liver, spleen and thymus.