Indirect photometric detection of anions in capillary electrophoresis
Indirect photometric detection of anions in capillary electrophoresis
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DOI：
10.1016/s0021-9673(98)00787-0
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发表时间：
1999-02
影响因子：
4.1
通讯作者：
P. Doble;P. Haddad
中科院分区：
文献类型：
--
作者：
P. Doble;P. Haddad
综述了毛细管电泳间接光度法检测阴离子的方法（吸光度法和荧光法）。讨论了影响分析物离子在含有一个或多个共离子的背景电解质中位移过程的因素，概述了间接检测模式下影响检测灵敏度的因素。本文介绍了用间接吸光度法测定阴离子时缓冲背景电解质的方法，并讨论了间接检测阴离子的背景电解质组成的设计和优化方法。然后综述了间接检测在主要分析物群分离中的应用，包括无机阴离子、有机酸、碳水化合物、磷酸盐、磷酸盐和杂种。提供了用于间接检测阴离子的探针的物种的物理性质的详细信息。
Indirect photometric detection (absorbance and fluorescence) of anions by capillary electrophoresis is reviewed. Factors which influence the displacement process of analyte ions in background electrolytes containing one or more co-ions are discussed and factors which influence detection sensitivity in indirect detection modes are outlined. Procedures are presented for buffering background electrolytes when indirect absorbance detection is to be used in the determination of anions, and approaches to the design and optimization of the composition of background electrolytes for indirect detection of anions are discussed. The application of indirect detection to the separation of major groups of analytes including inorganic anions, organic acids, carbohydrates, phosphates, phosphonates and miscellaneous species is then reviewed. Detailed information on the physical properties of species used as probes for indirect detection of anions is provided.