DJ-1, a causative gene product of a familial form of Parkinson's disease, is secreted through microdomains
DJ-1, a causative gene product of a familial form of Parkinson's disease, is secreted through microdomains
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DOI：
10.1016/j.febslet.2008.06.043
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发表时间：
2008-07-23
期刊：
影响因子：
3.5
通讯作者：
Ariga, Hiroyoshi
中科院分区：
文献类型：
--
作者：
Tsuboi, Yumi;Munemoto, Haruko;Ariga, Hiroyoshi
DJ-1可分泌到多种疾病患者的血清和血浆中。本研究发现DJ-1可以分泌到多种细胞的培养基中，在106 （C106）时，野生型DJ-1的分泌量是半胱氨酸突变型DJ-1的2倍。此外，HeLa细胞分泌的DJ-1中超过90%的氧化状态是SOH和SO2H形式的C106。细胞中DJ-1的一部分定位于膜的微域。这些发现表明，DJ-1是通过微结构域分泌的，DJ-1在C106位点的氧化促进了分泌。(C) 2008年欧洲生化学会联合会。Elsevier b.v.版权所有。
DJ-1 is secreted into the serum and plasma of patients with various diseases. In this study, DJ-1 was found to be secreted into culture media of various cells and the amount of wild-type DJ-1 secreted was two-fold greater than that of mutant DJ-1 of cysteine at 106 (C106). Furthermore, the oxidative status of more than 90% of the DJ-1 secreted from HeLa cells was SOH and SO2H forms of C106. A portion of DJ-1 in cells was localized in microdomains of the membrane. These findings suggest that DJ-1 is secreted through microdomains and that oxidation of DJ-1 at C106 facilitates the secretion. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.