Pulse-mode scanning ion conductance microscopy - a method to investigate cultured hippocampal cells
Pulse-mode scanning ion conductance microscopy - a method to investigate cultured hippocampal cells
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DOI：
10.1016/s0165-0270(02)00023-7
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发表时间：
2002-05-15
影响因子：
3
通讯作者：
Dietzel, ID
中科院分区：
文献类型：
--
作者：
Mann, SA;Hoffmann, G;Dietzel, ID
扫描离子电导显微镜（SICM）利用了玻璃微电极靠近导电不良的膜时电阻增加的优势（Science 243(1989) 641），已被证明是研究活细胞膜的一种很有前途的技术(Biophys J 73 (1997a) 653；[J] .中国生物医学工程学报，2008(5):391 - 391。基于倒置光学显微镜顶部的一种新设计装置，结合速度优化的低噪声细胞内放大器，提出了一种新的探针与表面距离控制模式。通过应用电流脉冲，可以独立于电极漂移和寄生直流电流来监测电阻的变化。我们通过展示脉冲模式操作的SICM (C) 2002 Elsevier Science B.V.产生的神经细胞的第一张高分辨率图像来证明其适用性。
Scanning ion conductance microscopy (SICM) takes advantage of the increase in the resistance which occurs if a glass microelectrode is closely approached to a poorly conducting membrane (Science 243 (1989) 641) and has been shown to be a promising technique to study membranes of living cells (Biophys J 73 (1997a) 653; J Microsc 188 (1997b) 17). Based on a newly designed set-up on top of an inverted light microscope in combination with a speed optimized low noise intracellular amplifier, a novel mode for control of the distance between the probe and surface has been developed. By application of current pulses, the change in the resistance is monitored independently from electrode drift and parasitic DC currents. We demonstrate the applicability by showing first high-resolution images of neural cells produced with the pulse-mode operated SICM (C) 2002 Elsevier Science B.V. All rights reserved.