X-ray dark-field radiography facilitates the diagnosis of pulmonary fibrosis in a mouse model.
X-ray dark-field radiography facilitates the diagnosis of pulmonary fibrosis in a mouse model.
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DOI：
10.1038/s41598-017-00475-3
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发表时间：
2017-03-23
影响因子：
4.6
通讯作者：
Meinel FG
中科院分区：
文献类型：
--
作者：
Hellbach K;Yaroshenko A;Willer K;Conlon TM;Braunagel MB;Auweter S;Yildirim AÖ;Eickelberg O;Pfeiffer F;Reiser MF;Meinel FG
本研究的目的是评价X线暗视野摄影能否提高投影摄影对肺纤维化的诊断水平。用基于光栅式小动物扫描仪的样机采集了C57BL/6N小鼠的肺X射线透射率和暗场图像，分别用博莱霉素诱导的肺纤维化和PBS作对照。两位盲人读者，都是经验丰富的放射科医生，熟悉暗视野成像，必须评估暗视野和传输图像是否存在纤维化。此外，读者还被要求对他们的诊断信心阶段进行评分。肺组织学评价作为本研究的参考标准。在分析暗视野X线片时，两位读者都表现出明显更高的诊断信心(p <； 0.001)。与单独分析吸收图像相比，在单独评估暗视野图像(p = 0.02)或结合透射性图像(p = 0.01)评估肺部时，诊断准确率显著提高。读者之间的一致性从仅评估传输图像时的良好提高到单独分析暗视野图像或与传输图像结合时的优秀。在小鼠肺纤维化模型中，将暗视野图像添加到传统的透射图像中，可以在胸片上改善对这种疾病的诊断。
The aim of this study was to evaluate whether diagnosing pulmonary fibrosis with projection radiography can be improved by using X-ray dark-field radiograms. Pulmonary X-ray transmission and dark-field images of C57Bl/6N mice, either treated with bleomycin to induce pulmonary fibrosis or PBS to serve as controls, were acquired with a prototype grating-based small-animal scanner. Two blinded readers, both experienced radiologists and familiar with dark-field imaging, had to assess dark-field and transmission images for the absence or presence of fibrosis. Furthermore readers were asked to grade their stage of diagnostic confidence. Histological evaluation of the lungs served as the standard of reference in this study. Both readers showed a notably higher diagnostic confidence when analyzing the dark-field radiographs (p < 0.001). Diagnostic accuracy improved significantly when evaluating the lungs in dark-field images alone (p = 0.02) or in combination with transmission images (p = 0.01) compared to sole analysis of absorption images. Interreader agreement improved from good when assessing only transmission images to excellent when analyzing dark-field images alone or in combination with transmission images. Adding dark-field images to conventional transmission images in a murine model of pulmonary fibrosis leads to an improved diagnosis of this disease on chest radiographs.