A comparative study of an indirect fluorescent antibody test and an immunoperoxidase monolayer assay for the diagnosis of porcine proliferative enteropathy
A comparative study of an indirect fluorescent antibody test and an immunoperoxidase monolayer assay for the diagnosis of porcine proliferative enteropathy
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DOI：
10.1177/104063870201400512
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发表时间：
2002-09-01
影响因子：
1.5
通讯作者：
Mackie-Nuss, RA
中科院分区：
文献类型：
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作者：
Guedes, RMC;Gebhart, CJ;Mackie-Nuss, RA
比较了目前用于检测猪增殖性肠病（PPE）抗体的间接荧光抗体试验（IFAT）和免疫过氧化物酶单层试验（IPMA）。在第0天（实验前攻毒）和经口接种受PPE影响的猪（28头攻毒猪）和蔗糖磷酸盐谷氨酸盐溶液（2头对照猪）的肠匀浆后第7、14、21和28天，从5周龄猪中采集用于该比较的血清样品。接种后4周，对所有动物实施安乐死。采用免疫组织化学方法检测回肠石蜡包埋切片中胞内劳森氏菌抗原的表达。除第0天和第7天1只对照动物的血清学结果（IPMA为阳性，IFAT为阴性）外，所有样品中每种方法的血清学结果均一致。IFAT与IPMA的一致率为98.6%。
The currently used indirect fluorescent antibody test (IFAT) for the detection of antibodies against porcine proliferative enteropathy (PPE) was compared to an immunoperoxidase monolayer assay (IPMA). Serum samples used in this comparison were collected from 5-week-old pigs on day 0 (pre-experimental challenge) and on days 7, 14, 21, and 28 after oral inoculation with intestinal homogenate from pigs affected by PPE (28 challenged pigs) and sucrose phosphate glutamate solution (2 control pigs). All animals were euthanized 4 weeks after inoculation. Immunohistochemistry staining was applied to formalin-fixed, paraffin-embedded sections of ileum for the detection of Lawsonia intracellularis antigen. The serology results with each method agreed in all samples, except on days 0 and 7 in 1 control animal, which was positive by IPMA, but negative by IFAT. The percentage of agreement between IFAT and IPMA was 98.6%.