Regional and cellular localization of the CXC112/SDF-1 chemokine receptor CXCR7 in the developing and adult rat brain
Regional and cellular localization of the CXC112/SDF-1 chemokine receptor CXCR7 in the developing and adult rat brain
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DOI：
10.1002/cne.21780
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发表时间：
2008-09-10
影响因子：
2.5
通讯作者：
Stumm, Ralf
中科院分区：
文献类型：
--
作者：
Schoenemeier, Bastian;Kolodziej, Angela;Stumm, Ralf
趋化因子基质细胞衍生因子-1 （SDF-1）通过趋化因子受体CXCR4调控神经元发育。在成人大脑中，SDF-1/CXCR4系统与神经发生、神经调节、脑炎症、肿瘤生长和HIV脑病有关。在最近发现RDC1/CXCR7是第二个SDF-1受体之前，CXCR4被认为是SDF-1的唯一受体。在这里，我们提供了第一个CXCR7 mRNA在胚胎和成年大鼠脑中的表达图谱。在胚胎阶段，CXCR7和CXCR4共同分布在神经节突起、尾状壳核的萌发区，并沿gaba能前体向皮层迁移的路线分布。在皮质中，在gaba能前体和一些表达reelin的Cajal-Retzius细胞中发现了CXCR7。与CXCR4不同，CXCR7在形成皮质板的神经元中丰富，而在发育中的齿状回和小脑外生发层中较少。在成人大脑中，CXCR7在血管、CA3中的锥体细胞和成熟齿状回颗粒细胞中表达，这与SDF-1模式相似。CXCR7和CXCR4在四个心室壁重叠。其他表达CXCR7的神经元结构包括嗅球、伏隔壳、下丘脑视上核和腹内侧核、丘脑内侧核和脑干运动核。此外，glast表达的星形胶质细胞显示了CXCR7的信号。因此，在sdf -1依赖性神经元发育过程中，CXCR4和CXCR7可能相互合作或单独作用。在成熟的神经元和血管中，CXCR7似乎是优势的sdf -1受体。[j] .中华神经科学杂志，2008。(c) 2008 Wiley-Liss, Inc。
The chemokine stromal cell-derived factor-1 (SDF-1) regulates neuronal development via the chemokine receptor CXCR4. In the adult brain the SDF-1/CXCR4 system was implicated in neurogenesis, neuromodulation, brain inflammation, tumor growth, and HIV encephalopathy. Until the recent identification of RDC1/CXCR7 as the second SDF-1 receptor, CXCR4 was considered to be the only receptor for SDF-1. Here we provide the first map of CXCR7 mRNA expression in the embryonic and adult rat brain. At embryonic stages, CXCR7 and CXCR4 were codistributed in the germinative zone of the ganglionic eminences, caudate putamen, and along the routes of GABAergic precursors migrating toward the cortex. In the cortex, CXCR7 was identified in GABAergic precursors and in some reelin-expressing Cajal-Retzius cells. Unlike CXCR4, CXCR7 was abundant in neurons forming the cortical plate and sparse in the developing dentate gyrus and cerebellar external germinal layer. In the adult brain, CXCR7 was expressed by blood vessels, pyramidal cells in CA3, and mature dentate gyrus granule cells, which is reminiscent of the SDF-1 pattern. CXCR7 and CXCR4 overlapped in the wall of the four ventricles. Further neuronal structures expressing CXCR7 comprised the olfactory bulb, accumbens shell, supraoptic and ventromedial hypothalamic nuclei, medial thalamus, and brain stem motor nuclei. Also, GLAST-expressing astrocytes showed signals for CXCR7. Thus, CXCR4 and CXCR7 may cooperate or act independently in SDF-1-dependent neuronal development. In mature neurons and blood vessels CXCR7 appears to be the preponderant SDF-1-receptor. J. Comp. Neurol. 510:207-220, 2008. (c) 2008 Wiley-Liss, Inc.