Plastid DNA polymerases from higher plants, Arabidopsis thaliana
Plastid DNA polymerases from higher plants, Arabidopsis thaliana
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DOI：
10.1016/j.bbrc.2005.06.052
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发表时间：
2005-08-19
影响因子：
3.1
通讯作者：
Sakaguchi, K
中科院分区：
文献类型：
--
作者：
Mori, Y;Kimura, S;Sakaguchi, K
以前，我们描述了一种新的DNA聚合酶，命名为OsPolI-like，从水稻。OsPoll-like与蓝藻的DNA聚合酶I具有高度的序列同源性，并定位于质体中。在这里，我们描述了两个Poll样聚合酶，命名为AtPolI-like A和AtPolI-like B，从拟南芥。原位杂交分析表明，这两种mRNA的表达在增殖组织，如拍摄顶端分生组织。GFP融合蛋白的定位分析表明AtPolI-like A和AtPolI-like B定位于质体。AtPolI样B的表达可以通过暴露于诱变剂H2O2来诱导。这些结果表明AtPolI-like B在氧化诱导的DNA损伤修复中具有作用。我们的数据表明，高等植物具有两个质体DNA聚合酶，没有发现在动物和酵母。(c)2005年爱思唯尔公司All rights reserved.
Previously, we described a novel DNA polymerase, designated as OsPolI-like, from rice. The OsPoll-like showed a high degree of sequence homology with the DNA polymerase I of cyanobacteria and was localized in the plastid. Here, we describe two Poll-like polymerases, designated as AtPolI-like A and AtPolI-like B, from Arabidopsis thaliana. In situ hybridization analysis demonstrated expression of both mRNAs in proliferating tissues such as the shoot apical meristem. Analysis of the localizations of GFP fusion proteins showed that AtPolI-like A and AtPolI-like B were localized to plastids. AtPolI-like B expression could be induced by exposure to the mutagen H2O2. These results suggested that AtPolI-like B has a role in the repair of oxidation-induced DNA damage. Our data indicate that higher plants possess two plastid DNA polymerases that are not found in animals and yeasts. (c) 2005 Elsevier Inc. All rights reserved.