Diversity in methane enrichments from agricultural soil revealed by DGGE separation of PCR amplified 16S rDNA fragments
Diversity in methane enrichments from agricultural soil revealed by DGGE separation of PCR amplified 16S rDNA fragments
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DOI：
10.1111/j.1574-6941.1998.tb01557.x
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发表时间：
1998-05-01
影响因子：
4.2
通讯作者：
Torsvik, V
中科院分区：
文献类型：
--
作者：
Jensen, S;Ovreås, L;Torsvik, V
利用PCR扩增的16S rRNA基因V3区域的变性梯度凝胶电泳（DGGE）图谱来评估甲烷作为唯一碳源和能量源的富集培养的多样性。这种富集源于两种农业土壤。一种是有机含量低（10%）的沙质土壤，另一种是有机含量约50%的有机土壤。DGGE提供了可扩增序列类型分布的快速评估，表明每种土壤中都富集了特定的细菌种群。DGGE剖面显示，有机土壤中V3片段的扩增范围大于砂质土壤。对来自19个DGGE条带的片段进行测序，并与先前发表的27个16S rRNA基因序列进行比较。这些序列证实了高多样性，在每个富集中存在不同的甲基营养群体。在两种土壤类型的富集物中均发现了II型甲烷化菌序列，而与I型甲烷化菌序列无亲缘关系。有机土壤富集的部分片段与甲基营养体无关。大多数序列在cr-变形菌门的一个分支上远距离聚集。这些事实表明，以前描述的甲基营养体在农业土壤的甲烷富集中是丰富的。(C) 1998年欧洲微生物学会联合会。Elsevier Science B.V.出版
Denaturing gradient gel electrophoresis (DGGE) profiles of PCR amplified V3 regions of 16S rRNA genes were used to assess the diversity in enrichment cultures with methane as the only carbon and energy source. The enrichments originated from two agricultural soils. One was a sandy soil with low (10%) organic content, the other an organic soil with approximately 50% organic content. DGGE provided a fast evaluation of the distribution of amplifiable sequence types indicating that specific bacterial populations had been enriched from each soil. The DGGE profiles revealed a broader range of amplified V3 fragments in the community derived from organic soil than from sandy soil. Fragments from 19 individual DGGE bands were sequenced and compared with 27 previously published 16S rRNA gene sequences. The sequences confirmed the high diversity with the presence of different methylotrophic populations in each enrichment. No affiliation was found with type I methanotrophs, instead type II methanotroph sequences were found in the enrichments from both soil types. Some of the fragments from the organic soil enrichment were not affiliated with methylotrophs. Most of the sequences clustered distantly on a branch within the cr-Proteobacteria. These facts suggested that previously undescribed methylotrophs are abundant in methane enrichments from agricultural soil. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.