Resistance Mechanisms to an Acetolactate Synthase (ALS) Inhibitor in Water Starwort (Myosoton aquaticum) Populations from China
Resistance Mechanisms to an Acetolactate Synthase (ALS) Inhibitor in Water Starwort (Myosoton aquaticum) Populations from China
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DOI：
10.1614/ws-d-14-00184.1
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发表时间：
2015-10-01
期刊：
影响因子：
2.5
通讯作者：
Wang, Jinxin
中科院分区：
文献类型：
--
作者：
Liu, Weitang;Wu, Cuixia;Wang, Jinxin
对苯磺隆的过度依赖导致了水星属植物的抗药性进化。本研究对中国地区水星草种群对苯磺隆的抗性机制进行了研究。细胞色素P450单加氧酶(P450)抑制剂马拉硫磷增加了所有种群对苯磺隆的敏感性。敏感(S)群体JS24和抗性(R)群体JS16和JS17的除草剂剂量分别减少了2.3倍、2.5倍和4.1倍，GR(50)降低了50%。然而，R群体的GR(50)值仍远高于S群体。这一观察结果表明，P450介导的代谢增强是水星抗性的一种机制。苯磺隆可诱导所有供试种群的谷胱甘肽-S-转移酶(GST)活性。特别是，JS16的GST活性比JS17或JS24的GST活性更高，而且诱导更快。通过序列分析，确定了每个种群的抗性归因于突变的乙酰乳酸合成酶(ALS)等位基因。在JS16和JS17中检测到Pro197Ser替换。还开发了分子标记，以快速识别水星种群中携带特定Pro197Ser突变的抗性和个体。抗性图谱试验表明，R种群对硫代丁酸钠盐、氟乐灵、吡磺隆和氟卡巴隆表现出不同程度的抗性，而对咪草胺、氟比妥、2，4-D丁酯、异丙隆和敌草净敏感。本研究证实，水星属植物对除草剂的抗性与一种或至少两种抗性机制有关。命名：2，4-D丁酯；敌百虫；氟卡巴隆-Na；氟比妥-甲基丁酯；咪草胺；异丙隆；硫代巴比妥钠盐；吡磺隆；苯磺隆；水星草属。莫恩奇。
Overreliance on tribenuron has resulted in resistance evolution in water starwort. This study investigates the resistance mechanisms to tribenuron in water starwort populations from China. The cytochrome P450 monooxygenase (P450) inhibitor malathion increased tribenuron sensitivity in all populations. The decrease in the amount of herbicide dose that causes 50% growth reduction (GR(50)) for the sensitive (S) population JS24 and the resistant (R) populations JS16 and JS17 were 2.3-, 2.5-, and 4.1-fold, respectively. However, the GR(50) values for the R populations were still much higher than those of the S population. This observation indicates that P450-mediated enhanced metabolism is one mechanism for resistance in water starwort. The glutathione-S-transferase (GST) activity could be induced by tribenuron for all tested populations. In particular, the GST activity of JS16 is inherently greater and is more rapidly induced than that of JS17 or JS24. Resistance attributed to mutant acetolactate synthase (ALS) alleles was identified by sequence analysis for each population. Pro197Ser substitution was detected in JS16 and JS17. Molecular markers were also developed to rapidly identify resistance as well as individuals carrying the specific Pro197Ser mutation in water starwort populations. The resistance patterns experiment revealed that the R populations exhibited different levels of resistance to pyrithiobac sodium salt, florasulam, pyroxsulam, and flucarbazone-Na; however, R populations were sensitive to imazethapyr, fluroxypyr-meptyl, 2,4-D butylate, isoproturon, and diflufenican. This study establishes that either one or at least two resistance mechanisms are involved in herbicide resistance in water starwort. Moreover, these mechanisms might contribute to the different levels of resistance to tribenuron among water starwort populations.Nomenclature: 2,4-D butylate; diflufenican; florasulam; flucarbazone-Na; fluroxypyr-meptyl; imazethapyr; isoproturon; pyrithiobac sodium salt; pyroxsulam; tribenuron; water starwort, Myosoton aquaticum (L.) Moench.