Phylogenetic diversity of Archaea in sediment samples from a coastal salt marsh
Phylogenetic diversity of Archaea in sediment samples from a coastal salt marsh
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DOI：
10.1128/aem.63.12.4729-4733.1997
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发表时间：
1997-12-01
影响因子：
4.4
通讯作者：
Embley, TM
中科院分区：
文献类型：
--
作者：
Munson, MA;Nedwell, DB;Embley, TM
使用古细菌特异性引物创建的 16S rRNA 基因文库对来自潮汐溪和邻近植物沼泽区域的盐沼沉积物样品中的古细菌进行采样，这两个区域均表现出活跃的产甲烷作用和硫酸盐还原作用。尽管有些序列与先前从海洋沉积物中分离出的产甲烷菌的序列密切相关，但没有一个序列与培养类群的参考序列相同。恢复了广泛的广古菌门序列，但没有恢复到来自甲烷球菌、甲烷杆菌或泉古菌门的序列。密切相关的序列簇很常见，并且通常包含来自两个位点的序列，表明两个样本中都存在一些相关的生物体。与产甲烷菌密切相关的序列的恢复，例如可以使用氢以外的底物的甲烷球菌和产甲烷菌，为已发表的假设提供了支持，即此类产甲烷菌可能在富含硫酸盐的沉积物中很重要，并确定了一些可能的候选物。还发现了与产甲烷菌（例如能够利用氢的Methanoculleus和Methanogenium）密切相关的序列，这与之前的抑制剂和过程测量结果一致，表明这些类群的活性水平较低。更令人惊讶的是，我们恢复了与来自不同嗜盐古菌的序列密切相关的多种序列，以及与最近通过PCR和探测显示在海洋样本中具有世界性分布的海洋II类古菌序列特别相关的一组不同序列。
The Archaea present in salt marsh sediment samples from a tidal creek and from an adjacent area of vegetative marshland, both of which showed active methanogenesis and sulfate reduction, were sampled by using 16S rRNA gene libraries created with Archaea-specific primers. None of the sequences were the same as reference sequences from cultured taxa, although some were closely related to sequences from methanogens previously isolated from marine sediments. A wide range of Euryarchaeota sequences were recovered, but no sequences from Methanococcus, Methanobacterium, or the Crenarchaeota were recovered. Clusters of closely related sequences were common and generally contained sequences from both sites, suggesting that some related organisms were present in both samples. Recovery of sequences closely related to those of methanogens such as Methanococcoides and Methanolobus, which can use substrates other than hydrogen, provides support for published hypotheses that such methanogens are probably important in sulfate-rich sediments and identifies some likely candidates. Sequences closely related to those of methanogens such as Methanoculleus and Methanogenium, which are capable of using hydrogen, were also discovered, in agreement with previous inhibitor and process measurements suggesting that these taxa are present at low levels of activity. More surprisingly, we recovered a variety of sequences closely related to those from different halophilic Archaea and a cluster of divergent sequences specifically related to the marine group II archaeal sequences recently shown by PCR and probing to have a cosmopolitan distribution in marine samples.