Targeted delivery of therapeutic agents to the mouse brain using a stereotactic-guided focused ultrasound device.
Targeted delivery of therapeutic agents to the mouse brain using a stereotactic-guided focused ultrasound device.
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DOI：
10.1016/j.xpro.2023.102132
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发表时间：
2023-03-17
期刊：
影响因子：
--
通讯作者：
Chen, Hong
中科院分区：
文献类型：
--
作者：
Hu, Zhongtao;Yang, Yaoheng;Ye, Dezhuang;Chen, Si;Gong, Yan;Chukwu, Chinwendu;Chen, Hong
聚焦超声（FUS）结合微泡介导血脑屏障（BBB）开放（FUS-BBBO）的现有方案在临床前研究中需要昂贵的超声设备和复杂的操作程序。我们开发了一种低成本，易于使用，精确的FUS设备，用于临床前研究的小动物模型。在这里，我们提供了一个详细的协议，用于构建FUS换能器，将换能器连接到立体定向框架，以实现精确的脑靶向，应用集成FUS设备在小鼠中执行FUS-BBBO，并评估FUS-BBBO结果。有关本方案使用和执行的完整详细信息，请参见Hu等人。（2022年）。构建低成本、易于使用和精确聚焦超声设备的方案将设备与立体定向框架集成以实现精确脑靶向的步骤在小鼠模型上构建的设备的高度精确脑靶向使用该设备在小鼠中进行脑药物递送的示例出版商说明：进行任何实验方案都需要遵守实验室安全和伦理的当地机构指南。聚焦超声（FUS）结合微泡介导血脑屏障（BBB）开放（FUS-BBBO）的现有方案在临床前研究中需要昂贵的超声设备和复杂的操作程序。我们开发了一种低成本，易于使用，精确的FUS设备，用于临床前研究的小动物模型。在这里，我们提供了一个详细的协议，用于构建FUS换能器，将换能器连接到立体定向框架，以实现精确的脑靶向，应用集成FUS设备在小鼠中执行FUS-BBBO，并评估FUS-BBBO结果。
Existing protocols of focused ultrasound (FUS) combined with microbubble-mediated blood-brain barrier (BBB) opening (FUS-BBBO) in preclinical research require expensive ultrasound equipment and complex operating procedures. We developed a low-cost, easy-to-use, and precise FUS device for small animal models in preclinical research. Here, we provide a detailed protocol for building the FUS transducer, attaching the transducer to a stereotactic frame for precise brain targeting, applying the integrated FUS device to perform FUS-BBBO in mice, and evaluating the FUS-BBBO outcome. For complete details on the use and execution of this protocol, please refer to Hu et al. (2022). Protocol for building a low-cost, easy-to-use, and precise focused ultrasound device Steps for integrating the device with a stereotactic frame for precise brain targeting Highly precise brain targeting of the built device on the mouse model Example of using this device to perform brain drug delivery in mice Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Existing protocols of focused ultrasound (FUS) combined with microbubble-mediated blood-brain barrier (BBB) opening (FUS-BBBO) in preclinical research require expensive ultrasound equipment and complex operating procedures. We developed a low-cost, easy-to-use, and precise FUS device for small animal models in preclinical research. Here, we provide a detailed protocol for building the FUS transducer, attaching the transducer to a stereotactic frame for precise brain targeting, applying the integrated FUS device to perform FUS-BBBO in mice, and evaluating the FUS-BBBO outcome.
DOI：
10.1109/tbme.2022.3150781
发表时间：
2022-09
期刊：
IEEE transactions on bio-medical engineering
影响因子：
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作者：
通讯作者：
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影响因子：
4.6
作者：
Hu, Zhongtao;Yang, Yaoheng;Chen, Hong
通讯作者：
Chen, Hong
影响因子：
11.1
作者：
Ye, Dezhuang;Yuan, Jinyun;Yang, Yaoheng;Yue, Yimei;Hu, Zhongtao;Fadera, Siaka;Chen, Hong
通讯作者：
Chen, Hong