Immunohistochemical Localization of Progesterone Receptor Isoforms in the Chick Pre‐follicular Ovary
Immunohistochemical Localization of Progesterone Receptor Isoforms in the Chick Pre‐follicular Ovary
复制标题
鸡卵泡前卵巢中孕酮受体亚型的免疫组织化学定位
DOI：
10.1111/j.1439-0264.2001.t01-1-0318.x
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Camacho‐Arroyo
中科院分区：
文献类型：
--
作者：
González‐Morán;Camacho‐Arroyo
用免疫组织化学方法研究了孕激素受体A和B亚型在鸡卵泡前卵巢不同类型细胞中的分布。将刚孵出的雏鸡处死，取出左侧卵巢，固定并包埋在旁质体中。采用间接免疫过氧化物酶技术，制备切片（5μm厚）用于检测孕酮受体亚型。结果表明，孕激素受体定位于卵巢皮质生殖上皮和生殖细胞的细胞核，以及卵巢髓质腔隙通道的间质细胞和上皮细胞。未分化的细胞没有孕酮受体。在所有细胞亚群中，孕激素受体B是主要表达的亚型。这些数据表明，孕激素受体亚型在鸡卵泡前卵巢中差异表达，该组织中的孕激素效应由孕激素受体B亚型介导。
The distribution of progesterone receptor A and B isoforms in different cell types of the chick pre‐follicular ovary was studied by immunohistochemistry. Newly hatched chicks were killed and the left ovary was removed, fixed and embedded in paraplast. Sections (5 μm thick) were made for the detection of progesterone receptor isoforms, using a technique of indirect immunoperoxidase. The results indicate that progesterone receptors were localized in the nuclei of germinal epithelium and germ cells of the ovarian cortex and in the interstitial and epithelial cells of the lacunar channels of the ovarian medulla. Undifferentiated cells did not present progesterone receptors. In all cell subpopulations progesterone receptor B was the predominantly expressed isoform. These data suggest that progesterone receptor isoforms are differentially expressed in the chick pre‐follicular ovary and that progesterone effects in this tissue are mediated by the progesterone receptor B isoform.
DOI：
10.1210/mend-1-8-517
发表时间：
1987
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
--
作者：
Conneely,OM;Dobson,AD;Tsai,MJ;Beattie,WG;Toft,DO;Huckaby,CS;Zarucki,T;Schrader,WT;O'Malley,BW
通讯作者：
O'Malley,BW