High expression of SETDB1 mediated by miR-29a-3p associates with poor prognosis and immune invasion in breast invasive carcinoma.
High expression of SETDB1 mediated by miR-29a-3p associates with poor prognosis and immune invasion in breast invasive carcinoma.
复制标题
由miR-29a-3p辅助介导的SETDB1的高表达，预后不良和免疫侵袭。
DOI：
10.21037/tcr-21-1527
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发表时间：
2021-12
影响因子：
0.9
通讯作者：
Gao J
中科院分区：
文献类型：
--
作者：
Chen X;Li X;Wei C;Zhao C;Wang S;Gao J
乳腺浸润性癌（BRCA）预后较差。大量研究表明SET结构域分叉组蛋白赖氨酸甲基转移酶1 （SETDB1）参与了许多癌症的发生和发展。本研究旨在揭示SETDB1在BRCA发生发展中的潜在机制。利用ONCOMINE数据库、TIMER数据库、UALCAN数据库和GEPIA数据库分析SETDB1在人类肿瘤中的表达。我们利用实时定量聚合酶链反应（qPCR）检测SETDB1在细胞系中的表达水平，并在PrognoScan和Kaplan-Meier绘图仪网站上进行SETDB1的存活分析。上游调节器数据来源于starBase数据库。我们证实了SETDB1信使RNA （mRNA）水平在乳腺细胞系中高表达，并且我们还发现SETDB1在许多类型的癌症中高表达。此外，在BRCA中，SETDB1过表达与预后不良呈正相关。此外，我们首次预测miR-29a-3p是BRCA中SETDB1的潜在上游调节因子。我们的研究结果表明，SETDB1可能通过增加免疫细胞的浸润和影响免疫检查点的表达来发挥致癌作用。本研究提示miR-29a-3p可介导预后不良的SETDB1在BRCA中的表达和肿瘤免疫浸润。
Breast invasive carcinoma (BRCA) has a poor prognosis. Numerous studies have shown that SET domain bifurcated histone lysine methyltransferase 1 (SETDB1) is involved in the initiation and progression of many cancers. This study aims to reveal the potential mechanism of SETDB1 in the development and progression of BRCA. The ONCOMINE database, TIMER database, UALCAN database and GEPIA database were used to analyze the expression of SETDB1 in human cancers. We evaluated the expression level of SETDB1 in cell lines by quantitative real-time polymerase chain reaction (qPCR), and the survival analysis of SETDB1 was performed on PrognoScan and Kaplan-Meier plotter websites. The upstream regulator was obtained from starBase database. We confirmed that SETDB1 messenger RNA (mRNA) level showed high expression in breast cell lines, and we also found that SETDB1 showed high expression in many types of cancers. Moreover, SETDB1 overexpression was positively correlated with poor prognosis in BRCA. Furthermore, we first predicted miR-29a-3p was a potential upstream regulator of SETDB1 in BRCA. Our findings indicated that SETDB1 might play a carcinogenic role by increasing the infiltration of immune cell and influencing immune checkpoint expression. This study suggested that miR-29a-3p can mediate the expression of SETDB1 with poor prognosis and tumor immune infiltration in BRCA.