Lung responses in murine models of experimental asthma: Value of house dust mite over ovalbumin sensitization
Lung responses in murine models of experimental asthma: Value of house dust mite over ovalbumin sensitization
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DOI：
10.1016/j.resp.2017.09.001
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发表时间：
2018-01-01
影响因子：
2.3
通讯作者：
Habre, Walid
中科院分区：
文献类型：
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作者：
Doras, Camille;Petak, Ferenc;Habre, Walid
卵清蛋白 (OVA) 致敏在哮喘建模中存在局限性。因此，我们检查了使用纯化的天然过敏原屋尘螨 (HDM) 进行过敏致敏相对于使用 OVA 进行致敏的价值。对小鼠进行假处理，或以相同的时间顺序用 OVA 或 HDM 致敏。在对照条件下以及用醋甲胆碱 (MCh) 和特定过敏原刺激动物后，评估气道阻力、组织阻尼和弹性。对支气管肺泡灌洗液的炎症特征进行了表征，并进行了肺组织学检查。虽然肺部对特定过敏原的反应性没有差异，但仅在 HDM 致敏动物的肺外周参数中观察到对 MCh 的高反应性。不同模型之间的肺部炎症有所不同，但过度的支气管平滑肌重塑仅发生在 OVA 中。总之，我们证明纯化的天然过敏原通过表达这种慢性炎症性疾病的肺功能和结构的关键特征，提供了更相关的人类过敏性哮喘小鼠模型。
Ovalbumin (OVA) sensitization has limitations in modelling asthma. Thus, we examined the value of allergic sensitization using a purified natural allergen, house dust mite (HDM), over the sensitization performed with OVA. Mice were sham-treated, or sensitized with OVA- or HDM with identical chronology. Airway resistance, tissue damping and elastance were assessed under control conditions and after challenging the animals with methacholine (MCh) and the specific allergen. Inflammatory profile of the bronchoalveolar lavage fluid was characterized and lung histology was performed. While no difference in the lung responsiveness to the specific allergen was noted, hyperresponsiveness to MCh was observed only in the HDM-sensitized animals in the lung peripheral parameters. Lung inflammation differed between the models, but excessive bronchial smooth muscle remodelling occurred only with OVA. In conclusion, we demonstrate that a purified natural allergen offers a more relevant murine model of human allergic asthma by expressing the key features of this chronic inflammatory disease both in the lung function and structure.