A global phylogenetic analysis of Japanese tonsil-derived Epstein-Barr virus strains using viral whole-genome cloning and long-read sequencing
A global phylogenetic analysis of Japanese tonsil-derived Epstein-Barr virus strains using viral whole-genome cloning and long-read sequencing
复制标题
使用病毒全基因组克隆和长读长测序对日本扁桃体来源的 Epstein-Barr 病毒株进行全球系统发育分析
DOI：
10.1099/jgv.0.001549
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发表时间：
2021
影响因子：
3.8
通讯作者：
Kanda Teru
中科院分区：
文献类型：
--
作者：
Yajima Misako;Kakuta Risako;Saito Yutaro;Kitaya Shiori;Toyoda Atsushi;Ikuta Kazufumi;Yasuda Jun;Ohta Nobuo;Kanda Teru
EB病毒（EBV）在大多数健康个体中建立终身潜伏感染，同时它是多种疾病（包括一些恶性肿瘤）的病原体。最近的高通量测序结果表明，不同的EBV毒株之间存在相当程度的病毒基因组异质性。然而，无症状感染个体之间EBV毒株变异的程度仍然难以捉摸。在这里，我们提出了一个简化的实验策略，克隆和测序EBV基因组来自人类扁桃体组织，这是无症状EBV感染的水库。完整的EBV基因组序列，包括重复区域，确定了7扁桃体来源的EBV株。基于全世界非肿瘤来源的EBV毒株的全病毒基因组序列的系统发育分析显示，亚洲EBV毒株可分为几个不同的亚组。来自鼻咽癌流行区的EBV毒株构成了与来自非流行区（包括日本）的EBV毒株亚组不同的亚组。结果可能与EBV相关疾病的偏倚区域分布一致，这取决于亚洲国家不同地区的不同EBV毒株。
Epstein–Barr virus (EBV) establishes lifelong latent infection in the majority of healthy individuals, while it is a causative agent for various diseases, including some malignancies. Recent high-throughput sequencing results indicate that there are substantial levels of viral genome heterogeneity among different EBV strains. However, the extent of EBV strain variation among asymptomatically infected individuals remains elusive. Here, we present a streamlined experimental strategy to clone and sequence EBV genomes derived from human tonsillar tissues, which are the reservoirs of asymptomatic EBV infection. Complete EBV genome sequences, including those of repetitive regions, were determined for seven tonsil-derived EBV strains. Phylogenetic analyses based on the whole viral genome sequences of worldwide non-tumour-derived EBV strains revealed that Asian EBV strains could be divided into several distinct subgroups. EBV strains derived from nasopharyngeal carcinoma-endemic areas constitute different subgroups from a subgroup of EBV strains from non-endemic areas, including Japan. The results could be consistent with biased regional distribution of EBV-associated diseases depending on the different EBV strains colonizing different regions in Asian countries.