Localization of relaxin in endometrial gland cells of pregnant, lactating, and ovariectomized hormone-treated guinea pigs.
Localization of relaxin in endometrial gland cells of pregnant, lactating, and ovariectomized hormone-treated guinea pigs.
复制标题
松弛素在妊娠、哺乳和卵巢切除激素处理的豚鼠子宫内膜腺细胞中的定位。
作者：
R. Pardo;L. Larkin
子宫取自豚鼠在不同阶段的怀孕和哺乳期间，以及动物卵巢切除和激素治疗，检查松弛素与过氧化物酶-抗过氧化物酶（PAP）技术采用抗血清产生的猪松弛素。虽然在妊娠第15天动物的子宫中未检测到松弛素，但在妊娠第30天动物的少数子宫内膜腺体中观察到少量松弛素。子宫从第45天，第60天，妊娠后期的动物表现出大量的松弛素，然而，子宫内膜腺细胞从泌乳动物含有很少或没有松弛素。注射雌激素和孕激素可以使卵巢切除的豚鼠的骨盆韧带松弛，也可以诱导松弛素在子宫内膜腺体中的积累。在子宫的非子宫内膜部分、子宫内膜间质成分、腔表面上皮或子宫颈腺体中未检测到松弛素。在小鼠子宫生物测定中，从第45天、第60天和晚期妊娠动物的子宫制备的提取物显示松弛素阳性反应。
Uteri taken from guinea pigs in different stages of pregnancy and during lactation, as well as animals that had been ovariectomized and treated with hormones, were examined for relaxin with the peroxidase-antiperoxidase (PAP) technique employing an antiserum produced against porcine relaxin. While relaxin was not detected in uteri of day 15 pregnant animals, small amounts were noted in a few endometrial glands in day 30 pregnant animals. Uteri from day 45, day 60, and late-pregnant animals exhibited heavy amounts of relaxin; however, endometrial gland cells from lactating animals contained very little or no relaxin. Injections of estrogen and progesterone that brought a relaxation of the pelvic ligaments in ovariectomized guinea pigs also induced an accumulation of relaxin in the endometrial glands. Relaxin was not detected in nonendometrial portions of the uterus, endometrial stromal components, luminal surface epithelium, or uterine cervical glands. Extracts prepared from uteri of day 45, day 60, and late-pregnant animals showed positive responses for relaxin in the mouse uterus bioassay.