High sensitivity measurement of estrone and estradiol in serum and plasma using LC-MS/MS.
High sensitivity measurement of estrone and estradiol in serum and plasma using LC-MS/MS.
复制标题
使用 LC-MS/MS 高灵敏度测量血清和血浆中的雌酮和雌二醇。
作者：
M. Kushnir;A. Rockwood;Bingfang Yue;A. Meikle
测量低浓度的雌激素，在青春期前的儿童，男性和绝经后的妇女，是重要的许多临床应用。我们描述了一种使用二维色谱分离和串联质谱检测的雌激素高灵敏度分析方法。将血清或血浆样品的等分试样与稳定同位素标记的内标物合并，并用甲基叔丁基醚提取雌激素。蒸发溶剂，雌激素衍生形成丹磺酰衍生物，并分析样品。使用配备电喷雾离子源的三重四极杆质谱仪进行定量，采用正离子模式电离和多反应监测采集。
Measurement of low concentrations of estrogens, encountered in pre-pubertal children, men, and postmenopausal women, is important for numerous clinical applications. We describe a method for high sensitivity analysis of estrogens that uses two-dimensional chromatographic separation and tandem mass spectrometry detection. Aliquots of serum or plasma samples are combined with stable isotope-labeled internal standard and estrogens are extracted with methyl t-butyl ether. The solvent is evaporated, estrogens derivatized to form dansyl derivatives, and the samples are analyzed. Quantitation is performed using triple quadrupole mass spectrometer equipped with electrospray ion source using positive ion mode ionization and multiple reaction monitoring acquisition.
影响因子：
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作者：
Sarnak, Mark J.;Katz, Ronit;Shlipak, Michael G.
通讯作者：
Shlipak, Michael G.