Photo-acceleration of protein release from endosome in the protein transduction system
Photo-acceleration of protein release from endosome in the protein transduction system
复制标题
DOI：
10.1016/j.febslet.2004.07.033
复制
发表时间：
2004-08-13
期刊：
影响因子：
3.5
通讯作者：
Matsui, H
中科院分区：
文献类型：
--
作者：
Matsushita, M;Noguchi, H;Matsui, H
蛋白质转导系统已被用于通过内吞机制将生物活性蛋白质递送到细胞中。然而，内吞蛋白在内体中的捕获可显著减弱细胞中的生物学作用。本研究表明，暴露于荧光可以人为地加速转导蛋白质的内体释放。暴露于480 nm的光刺激转导的FITC-11精氨酸-蛋白转导结构域（11 R-PTD）、TAT-PTD和拟南芥-PTD的内体释放。此外，FITC-11 R-p53蛋白在光刺激后从内体释放。这些数据表明，光加速是一个更有效的策略，在蛋白质转导系统。(C)2004年由Elsevier B. V.代表欧洲生物化学学会联合会出版。
The protein transduction system has been employed for delivery of bioactive proteins into cells via an endocytotic mechanism. However, trapping of endocytosed proteins in the endosome may significantly attenuate biological actions in cells. The present investigation demonstrated that endosomal release of transduced protein could be artificially accelerated by exposure to fluorescent light. Exposure to light at 480 nm stimulated endosomal release of transduced FITC-11 arginine-protein transduction domain (11R-PTD), TAT-PTD and Antennapedia-PTD. Moreover, FITC-11R-p53 protein was released from endosomes following stimulation with light. These data suggest that photo-acceleration is a more efficient strategy in terms of the protein transduction system. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.