The protective effects of glutamine in a rat model of ventilator-induced lung injury
The protective effects of glutamine in a rat model of ventilator-induced lung injury
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DOI：
10.3978/j.issn.2072-1439.2014.11.06
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发表时间：
2014-12-01
影响因子：
2.5
通讯作者：
Zhang, Haibo
中科院分区：
文献类型：
--
作者：
Chen, Chin-Ming;Cheng, Kuo-Chen;Zhang, Haibo
背景资料：急性呼吸窘迫综合征（ARDS）患者尽管采取了保护性治疗措施，但死亡率仍然很高。我们试图研究谷氨酰胺（GLN）在内毒素诱导的炎症和呼吸机诱导的肺损伤（VILI）的两次打击模型中的药理学作用。我们假设给予GLN可以改善VILI。将Sprague-Dawley大鼠麻醉并经气管内给予脂多糖（LPS）作为第一次打击以诱导肺部炎症，24小时后，第二次机械通气（MV），采用低潮气量（6 mL/kg）和5 cmH 2 O呼气末正压（PEEP）或高潮气量（22 mL/kg），零PEEP持续4 h。GLN或乳酸林格氏溶液作为安慰剂静脉注射给药前15分钟MV。结果：在高潮气量的LPS-激发的大鼠通气，GLN治疗改善肺损伤指数，肺力学和细胞因子反应与安慰剂组相比。结论：GLN的管理之前立即MV可能是有益的，在减少VILI的背景下。
Background: The mortality rate of patients with acute respiratory distress syndrome (ARDS) is still high despite the use of protective ventilatory strategies. We sought to examine the pharmacological effects of glutamine (GLN) in a two-hit model of endotoxin-induced inflammation followed by ventilator-induced lung injury (VILI). We hypothesized that the administration of GLN ameliorates the VILI.Methods: Sprague-Dawley rats were anesthetized and given lipopolysaccharide (LPS) intratracheally as a first hit to induce lung inflammation, followed 24 h later by a second hit of mechanical ventilation (MV) with either low tidal volume (6 mL/kg) with 5 cmH(2)O of positive end-expiratory pressure (PEEP) or high tidal volume (22 mL/kg) with zero PEEP for 4 h. GLN or lactated Ringer's solution as the placebo was administered intravenously 15 min prior to MV.Results: In the LPS-challenged rats ventilated with high tidal volume, the treatment with GLN improved lung injury indices, lung mechanics and cytokine responses compared with the placebo group.Conclusions: The administration of GLN given immediately prior to MV may be beneficial in the context of reducing VILI.