Cloning, structural analysis and expression of the gene encoding aspartate aminotransferase from the thermophilic cyanobacterium Phormidium lapideum
Cloning, structural analysis and expression of the gene encoding aspartate aminotransferase from the thermophilic cyanobacterium Phormidium lapideum
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DOI：
10.1263/jbb.95.421
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发表时间：
2003-04-01
影响因子：
2.8
通讯作者：
Sawa, Y
中科院分区：
文献类型：
--
作者：
Kim, H;Nakaoka, M;Sawa, Y
克隆了嗜热蓝藻lapormidium的天冬氨酸转氨酶基因，并在大肠杆菌中进行了表达。由1167个核苷酸组成的ORF编码了一个388个氨基酸的蛋白质，分子量为42099。PlAspAT的分子模型显示出与嗜热热菌相似的结构特征。
The aspartate aminotransferase gene from the thermophilic cyanobacterium Phormidium lapideum was cloned and expressed in Escherichia coli. The ORF of 1167 nucleotides encodes a protein of 388 amino acids having a molecular weight of 42,099. A molecular model of PlAspAT shows structural features similar to those of the Thermus thermophilus AspAT.